Cross-Sectional Evaluation of Humoral Responses against SARS-CoV-2 Spike.

Abstract

SARS-CoV-2 is responsible for the coronavirus disease 2019 (COVID-19) pandemic, infecting millions of people and causing hundreds of thousands of deaths. The Spike glycoproteins of SARS-CoV-2 mediate viral entry and are the main targets for neutralizing antibodies. Understanding the antibody response directed against SARS-CoV-2 is crucial for the development of vaccine, therapeutic, and public health interventions. Here, we perform a cross-sectional study on 106 SARS-CoV-2-infected individuals to evaluate humoral responses against SARS-CoV-2 Spike. Most infected individuals elicit anti-Spike antibodies within 2 weeks of the onset of symptoms. The levels of receptor binding domain (RBD)-specific immunoglobulin G (IgG) persist over time, and the levels of anti-RBD IgM decrease after symptom resolution. Although most individuals develop neutralizing antibodies within 2 weeks of infection, the level of neutralizing activity is significantly decreased over time. Our results highlight the importance of studying the persistence of neutralizing activity upon natural SARS-CoV-2 infection.

• Authors: Jérémie Prévost, Romain Gasser, Guillaume Beaudoin-Bussières, Jonathan Richard, Ralf Duerr, Annemarie Laumaea, Sai Priya Anand, Guillaume Goyette, Mehdi Benlarbi, Shilei Ding, Halima Medjahed, Antoine Lewin, Josée Perreault, Tony Tremblay, Gabrielle Gendron-Lepage, Nicolas Gauthier, Marc Carrier, Diane Marcoux, Alain Piché, Myriam Lavoie, Alexandre Benoit, Vilayvong Loungnarath, Gino Brochu, Elie Haddad, Hannah D Stacey, Matthew S Miller, Marc Desforges, Pierre J Talbot, Graham T Gould Maule, Marceline Côté, Christian Therrien, Bouchra Serhir, Renée Bazin, Michel Roger, Andrés Finzi
• Journal: Cell reports. Medicine (Cell Rep Med) Vol. 1 Issue 7 Pg. 100126 (10 20 2020) ISSN: 2666-3791 [Electronic] United States
• PMID: 33015650 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural)
• Copyright: © 2020 The Author(s).