Ishophloroglucin A Isolated from Ishige okamurae Suppresses Melanogenesis Induced by α-MSH: In Vitro and In Vivo.

Abstract	Diphlorethohydroxycarmalol (DPHC) isolated from Ishige okamurae (IO) showed potential whitening effects against UV-B radiation. However, the components of IO as well as their molecular mechanism against α-melanocyte-stimulating hormone (α-MSH) have not yet been investigated. Thus, this study aimed to investigate the inhibitory effects of Ishophloroglucin A (IPA), a phlorotannin isolated from brown algae IO, and its crude extract (IOE), in melanogenesis in vivo in an α-MSH-induced zebrafish model and in B16F10 melanoma cells in vitro. Molecular docking studies of the phlorotannins were carried out to determine their inhibitory effects and to elucidate their mode of interaction with tyrosinase, a glycoprotein related to melanogenesis. In addition, morphological changes and melanin content decreased in the α-MSH-induced zebrafish model after IPA and IOE treatment. Furthermore, Western blotting results revealed that IPA upregulated the extracellular related protein expression in α-MSH-stimulated B16F10 cells. Hence, these results suggest that IPA isolated from IOE has a potential for use in the pharmaceutical and cosmetic industries.
Authors	Xining Li, Hye-Won Yang, Yunfei Jiang, Jae-Young Oh, You-Jin Jeon, Bomi Ryu
Journal	Marine drugs (Mar Drugs) Vol. 18 Issue 9 (Sep 17 2020) ISSN: 1660-3397 [Electronic] Switzerland
PMID	32957728 (Publication Type: Journal Article)
Chemical References	Benzofurans Dioxins Melanins Skin Lightening Preparations ishophloroglucin A alpha-MSH Monophenol Monooxygenase JNK Mitogen-Activated Protein Kinases p38 Mitogen-Activated Protein Kinases
Topics	Animals Benzofurans (isolation & purification, pharmacology) Dioxins (isolation & purification, pharmacology) JNK Mitogen-Activated Protein Kinases (metabolism) Melanins (biosynthesis) Melanocytes (drug effects, metabolism, pathology) Melanoma, Experimental (metabolism, pathology) Mice Monophenol Monooxygenase (antagonists & inhibitors, metabolism) Phaeophyceae (metabolism) Phosphorylation Skin Lightening Preparations (isolation & purification, pharmacology) Zebrafish alpha-MSH (pharmacology) p38 Mitogen-Activated Protein Kinases (metabolism)

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