Abstract |
This study investigated the effects of a physiologically active peptide derived from rice bran (KF-8) on oxidative stress in d-galactose (d-gal)-induced aging mice and the underlying molecular mechanisms. The aging model was developed by subcutaneously injecting Institute of Cancer Research mice with 250 mg/kg d-gal daily for 12 weeks and simultaneously treating them with 30 mg/kg KF-8. The relative expression levels of Nrf2 and NF-κB in the liver were determined by the western blot. The regulation of Nrf2 and NF-κBp65 by KF-8 was further validated in NIH/3T3 cells. Compared with the control mice, the aging mice had significantly decreased body weights as well as superoxide dismutase and GSH-Px levels (p < 0.05); however, they had increased serum reactive oxygen species and malondialdehyde and 8-hydroxydeoxyguanosine levels accompanied by aortic and brain injuries. They also had elevated RAGE, TLR4, IκB, Bax, and caspase-8 expressions and NF-κB/p65 phosphorylation but reduced BcL-2 expression in the liver. Moreover, in vitro experiments demonstrated that the pretreatment of H2O2-treated NIH/3T3 cells with KF-8 significantly mitigated the NF-κB signaling and attenuated the Nrf2 nuclear transport (both p < 0.05). In conclusion, KF-8 treatment inhibited aging-induced oxidative stress-related organ injury in mice by attenuating NF-κB/p38 signaling and preserving Nrf2 activity.
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Authors | Yuqian Wang, Xiaoji Cui, Qinlu Lin, Jie Cai, Liuhuan Tang, Ying Liang |
Journal | Journal of agricultural and food chemistry
(J Agric Food Chem)
Vol. 68
Issue 44
Pg. 12271-12283
(Nov 04 2020)
ISSN: 1520-5118 [Electronic] United States |
PMID | 32942847
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- NF-E2-Related Factor 2
- NF-kappa B
- Peptides
- Reactive Oxygen Species
- Malondialdehyde
- Superoxide Dismutase
- Galactose
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Topics |
- Aging
(drug effects, metabolism)
- Animals
- Antioxidants
(administration & dosage)
- Apoptosis
(drug effects)
- Galactose
(adverse effects)
- Humans
- Male
- Malondialdehyde
(metabolism)
- Mice
- Mice, Inbred ICR
- NF-E2-Related Factor 2
(genetics, metabolism)
- NF-kappa B
(genetics, metabolism)
- Oryza
(chemistry)
- Oxidative Stress
(drug effects)
- Peptides
(administration & dosage)
- Reactive Oxygen Species
(metabolism)
- Seeds
(chemistry)
- Superoxide Dismutase
(metabolism)
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