Abstract | OBJECTIVE:
MicroRNAs ( miRNAs) may be viable targets for treating renal interstitial fibrosis (RIF). Fuzheng Huayu recipe (FZHY), a traditional Chinese compound herbal medicine, is often used in China to treat fibrosis. This study sought to assess the mechanisms through which FZHY influences miRNAs to treat RIF. METHODS: RIF was induced in rats by mercury chloride and treated with FZHY. Hydroxyproline content, Masson's staining and type I collagen expression were used to evaluate renal collagen deposition. Renal miRNA profiles were evaluated using a miRNA microarray. Those miRNAs that were differentially expressed following FZHY treatment were identified and subjected to bioinformatic analyses. The miR-21 target gene phosphatase and tensin homolog (PTEN) expression and AKT phosphorylation in kidney tissues were assessed via Western blotting. In addition, HK-2 human proximal tubule epithelial cells were treated using angiotensin II (Ang-II) to induce epithelial-to-mesenchymal transition (EMT), followed by FZHY exposure. miR-21 and PTEN expressions were evaluated via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), while E-cadherin and α-smooth muscle actin (α-SMA) expressions were assessed by immunofluorescent staining and qRT-PCR. Western blotting was used to assess PTEN and AKT phosphorylation. RESULTS: FZHY significantly decreased kidney collagen deposition, hydroxyproline content and type I collagen level. The miRNA microarray identified 20 miRNAs that were differentially expressed in response to FZHY treatment. Subsequent bioinformatic analyses found that miR-21 was the key fibrosis-related miRNA regulated by FZHY. FZHY also decreased PTEN expression and AKT phosphorylation in fibrotic kidneys. Results from in vitro tests also suggested that FZHY promoted E-cadherin upregulation and inhibited α-SMA expression in Ang-II-treated HK-2 cells, effectively reversing Ang-II-mediated EMT. We also determined that FZHY reduced miR-21 expression, increased PTEN expression and decreased AKT phosphorylation in these cells. CONCLUSION: miR-21 is the key fibrosis-related miRNA regulated by FZHY. The ability of FZHY to modulate miR-21/PTEN/AKT signaling may be a viable approach for treating RIF.
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Authors | Qing-Lan Wang, Yan-Yan Tao, Hong-Dong Xie, Cheng-Hai Liu, Ping Liu |
Journal | Journal of integrative medicine
(J Integr Med)
Vol. 18
Issue 6
Pg. 505-513
(Nov 2020)
ISSN: 2095-4964 [Print] Netherlands |
PMID | 32912827
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Shanghai Changhai Hospital. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Drugs, Chinese Herbal
- MicroRNAs
- fuzheng huayu
- mirn21 microRNA, rat
- Proto-Oncogene Proteins c-akt
- PTEN Phosphohydrolase
- Pten protein, rat
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Topics |
- Animals
- China
- Drugs, Chinese Herbal
(pharmacology)
- Fibrosis
- Kidney
(drug effects, pathology)
- MicroRNAs
- Nephritis, Interstitial
(drug therapy)
- PTEN Phosphohydrolase
- Proto-Oncogene Proteins c-akt
- Rats
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