A DNA nanodevice-based vaccine for cancer immunotherapy.

Abstract

A major challenge in cancer vaccine therapy is the efficient delivery of antigens and adjuvants to stimulate a controlled yet robust tumour-specific T-cell response. Here, we describe a structurally well defined DNA nanodevice vaccine generated by precisely assembling two types of molecular adjuvants and an antigen peptide within the inner cavity of a tubular DNA nanostructure that can be activated in the subcellular environment to trigger T-cell activation and cancer cytotoxicity. The integration of low pH-responsive DNA 'locking strands' outside the nanostructures enables the opening of the vaccine in lysosomes in antigen-presenting cells, exposing adjuvants and antigens to activate a strong immune response. The DNA nanodevice vaccine elicited a potent antigen-specific T-cell response, with subsequent tumour regression in mouse cancer models. Nanodevice vaccination generated long-term T-cell responses that potently protected the mice against tumour rechallenge.

• Authors: Shaoli Liu, Qiao Jiang, Xiao Zhao, Ruifang Zhao, Yuanning Wang, Yiming Wang, Jianbing Liu, Yingxu Shang, Shuai Zhao, Tiantian Wu, Yinlong Zhang, Guangjun Nie, Baoquan Ding
• Journal: Nature materials (Nat Mater) Vol. 20 Issue 3 Pg. 421-430 (03 2021) ISSN: 1476-4660 [Electronic] England
• PMID: 32895504 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Cancer Vaccines
• Vaccines, DNA

Topics

• Adjuvants, Immunologic (administration & dosage)
• Animals
• Antigen Presentation
• Bacteriophage M13 (genetics)
• Cancer Vaccines (administration & dosage, genetics, immunology)
• Cytotoxicity Tests, Immunologic
• Dendritic Cells (drug effects, immunology)
• Hydrogen-Ion Concentration
• Immunotherapy (methods)
• Lymphatic Metastasis (prevention & control)
• Lymphocytes, Tumor-Infiltrating (drug effects, immunology)
• Melanoma, Experimental (immunology, pathology, therapy)
• Mice, Inbred C57BL
• Vaccines, DNA (administration & dosage, genetics, immunology)
• Mice