Mite
allergens are considerable factors in the genesis of allergic diseases. The storage mite Tyrophagus putrescentiae (Tp) appears in contaminated foods and household surroundings. The current diagnostic tools for Tp
allergy are mostly based on
crude extracts and still contain shortcomings. This study aimed to investigate the
immunoglobulin E (
IgE)- responsiveness profiles of Tp-allergic patients and develop a molecular diagnostic method using recombinant
allergens. Allergenic components were characterized as cross-reacting or species-specific
allergens, in which the effective combinations of recombinant
allergens were developed and analyzed in terms of the prediction accuracy for clinical diagnosis. Seven recombinant
allergens were cloned and generated to detect the
IgE responsiveness of the Tp
allergy. A survey on the prevalence of mite
allergy showed there were higher sensitizations with
IgE responsiveness to house dust mites (HDM) (78.9-80.9%) than to storage mites Tp (35.6%). Prevalence of sensitization to Tp was higher in elderly subjects. The principal
IgE-binding components of Tp were Tyr p 1, Tyr
p 2 and Tyr p 3. Prediction accuracy for Tp
allergy by
IgE-responsiveness combination D (Tyr p 1, Tyr
p 2 & Tyr p 3) was with high precision (100%). Avoiding the cross-reactivity of Dermatophagoides pteronyssinus, the prediction accuracy of
IgE-responsiveness combination H+ (Tyr p 1, Tyr
p 2, Tyr p 3, Tyr p 7, Tyr p 8, Tyr p 10 & Tyr p 20) was suitable for Tp-specific diagnosis. Panels of Tp
allergens were generated and developed a diagnostic kit able beneficial to identify
IgE-mediated Tp
hypersensitivity.