Abstract |
In this study, three strains of lactobacilli and bifidobacteria originally isolated from healthy infants, were tested for their abilities to activate RAW264.7 cells. Gene expression and cytokine production of interleukin-10 (IL-10) of RAW264.7 cells were evaluated. The activation of extracellular regulated protein kinases 1/2 (ERK1/2), p38, and nuclear factor-κB (NK-κB) were also assessed. These results suggest lactobacilli and bifidobacteria in infants may promote production of IL-10 in macrophages, conferring a protective effect in hosts suffering from inflammation. Dimerization of TLR2 and MyD88 and subsequent phosphorylation of the key downstream signaling molecules, such as MAPKs and NK-κB, may be one of the key underlying mechanisms of activation of macrophages by these microbes. Bifidobacteria and lactobacilli induced macrophages to secrete IL-10 in a different manner, which may relate to their abilities to activate key signaling pathways mediated by TLR2 and MyD88.
|
Authors | Huijing Liang, Zihao Luo, Zhonghua Miao, Xi Shen, Ming Li, Xuguang Zhang, Jiehua Chen, Xiaolei Ze, Qiwei Chen, Fang He |
Journal | Bioscience, biotechnology, and biochemistry
(Biosci Biotechnol Biochem)
Vol. 84
Issue 12
Pg. 2558-2568
(Dec 2020)
ISSN: 1347-6947 [Electronic] England |
PMID | 32862788
(Publication Type: Journal Article)
|
Chemical References |
- Myeloid Differentiation Factor 88
- Toll-Like Receptor 2
- Interleukin-10
|
Topics |
- Animals
- Bifidobacterium
(physiology)
- Humans
- Infant
- Interleukin-10
(metabolism)
- Intestines
(microbiology)
- Lactobacillus
(physiology)
- Macrophage Activation
- Mice
- Myeloid Differentiation Factor 88
(metabolism)
- RAW 264.7 Cells
- Signal Transduction
- Toll-Like Receptor 2
(metabolism)
|