Abstract | BACKGROUND: METHODS: A rat hepatocellular carcinoma (HCC) models was established by N-nitrosodiethylamine. Forty-eight of them were randomly divided into 3 equal groups: surgery without postoperative analgesia (Group C), surgery with morphine postoperative analgesia (Group M), surgery with sufentanil postoperative analgesia (Group S). Each animal underwent a standard left hepatolobectomy, and intraperitoneally implanted with osmotic minipumps filled with sufentanil, morphine or normal saline according to the different group. The food and water consumptions, body weight changes, locomotor activity and mechanical pain threshold (MPT) were observed. The ratio of CD4+/CD8+, proportions of Th1, Th2, Th17 and Treg cells in blood were detected using flow cytometry. The liver function and the rats' survival situation of each group were observed. RESULTS: The food and water consumption, locomotor activity and MPT of group C declined than those of group S and M on d1, d2, d3 (P < 0.05). The CD4+/CD8+ ratio and the proportion of Th1 cells were significantly higher while the proportion of Th2, Th17 and Treg cells were significantly lower in group S and group M compared with group C. The rats of group S have higher CD4+/CD8+ ratio on d3, while lower proportion of Treg cells on d7 compared with group M. The plasma ALT and AST values in group C were significantly higher than that of group S and group M on both d3 and d7. There were not significant differences in mortality rate between 3 groups. CONCLUSIONS:
|
Authors | Yanhua Peng, Jinfeng Yang, Duo Guo, Chumei Zheng, Huiping Sun, Qinya Zhang, Shuangfa Zou, Yanping Zhang, Ke Luo, Keith A Candiotti |
Journal | BMC anesthesiology
(BMC Anesthesiol)
Vol. 20
Issue 1
Pg. 212
(08 26 2020)
ISSN: 1471-2253 [Electronic] England |
PMID | 32847505
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Analgesics, Opioid
- Sufentanil
|
Topics |
- Analgesics, Opioid
(administration & dosage)
- Animals
- Carcinoma, Hepatocellular
(immunology, surgery)
- Liver Neoplasms
(immunology, surgery)
- Male
- Pain Threshold
(drug effects, physiology)
- Pain, Postoperative
(immunology, prevention & control)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Sufentanil
(administration & dosage)
- T-Lymphocytes, Regulatory
(drug effects, immunology)
- Th17 Cells
(drug effects, immunology)
|