BACKGROUND
Atherosclerosis is a progressive inflammatory disease that involves a variety of inflammatory and proinflammatory factors, including
intercellular adhesion molecule (ICAM)-1.
ICAM-1 plays an important role in
atherosclerosis by promoting cell adhesion. Mixed lineage
kinase domain-like (MLKL), a critical regulator of necroptotic cell death, is indicated to play an important role in
atherosclerosis. This study investigated the effects of MLKL on
ICAM-1 expression and cell adhesion, thus providing a new direction for the research of
atherosclerosis pathogenesis. MATERIAL AND METHODS
siRNA-MLKL and pcDNA-MLKL were designed, and the expression of MLKL and
ICAM-1 were estimated by real-time polymerase chain reaction at the
mRNA level and Western blotting at the
protein level. The adhesion of human monocyte cells (THP-1) to human umbilical vein endothelial cells (HUVECs) was examined under immunofluorescence microscopy, and the ability of cell adhesion was evaluated by ImageJ software. RESULTS Overexpression of MLKL greatly enhanced
ICAM-1 expression in HUVECs and the adherence of THP-1 cells to HUVECs. Knockdown of MLKL by
siRNA dramatically inhibited the expression of
ICAM-1 and the adherence of THP-1 cells to HUVECs. MLKL could promote THP-1 adhesion to HUVECs by activating
ICAM-1 expression in HUVECs. CONCLUSIONS MLKL can promote THP-1 cell adhesion to HUVECs through up-regulation of
ICAM-1 expression in HUVECs. Thus, MLKL might be a useful target for reducing adhesion of monocytes to endothelial cells and
atherosclerosis.