HIPRO: A High-Efficiency, Hypoxia-Induced Protocol for Generation of Photoreceptors in Retinal Organoids from Mouse Pluripotent Stem Cells.

Abstract

Mouse pluripotent stem cells can be efficiently differentiated into retinal organoids with polarized, laminated neural retina harboring all retinal cell types by the Hypoxia-Induced Generation of Photoreceptor in Retinal Organoids (HIPRO) protocol. In our recent publication, we modified the HIPRO protocol on the basis of comparative transcriptome analyses to facilitate photoreceptor biogenesis and maturation. Here, we provide a detailed protocol for efficient generation of retinal organoids from mouse pluripotent stem cells. For complete details on the use and execution of this protocol, please refer to (Chen et al., 2016, DiStefano et al., 2018, Brooks et al., 2019).

• Authors: Holly Y Chen, Ryan A Kelley, Anand Swaroop
• Journal: STAR protocols (STAR Protoc) Vol. 1 Issue 1 (06 19 2020) ISSN: 2666-1667 [Electronic] United States
• PMID: 32754720 (Publication Type: Journal Article, Research Support, N.I.H., Intramural)

Chemical References

• Culture Media

Topics

• Animals
• Culture Media
• Cytological Techniques
• Humans
• Mice
• Organoids (cytology)
• Photoreceptor Cells, Vertebrate (cytology)
• Pluripotent Stem Cells