Asthenozoospermia is one of the major causes of human
male infertility. Long noncoding RNAs (lncRNAs) play critical roles in the spermatogenesis processes. The present study aims to investigate the intricate regulatory network associated with
asthenozoospermia. The lncRNAs expression profile was analyzed in the
asthenozoospermia seminal plasma exosomes by
RNA-sequencing, and the functions of differentially expressed genes (DEGs) were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and DO (Disease Ontology) enrichment analyses. Pearson's correlation test was utilized to calculate the correlation coefficients between
lncRNA and mRNAs. Moreover, the
lncRNA-
miRNA-
mRNA co-expression network was constructed with bioinformatics. From the co-expression analyses, we identified the cis regulated correlation pairs
lncRNA-
mRNA. To confirm sequencing results with five of the identified DElncRNAs were verified with quantitative reverse-transcription polymerase chain reaction (qRT-PCR). We identified 4228 significantly DEGs, 995 known DElncRNAs, 2338 DEmRNAs and 11,706 novel DElncRNAs between
asthenozoospermia and normal group. GO and KEGG analyses showed that the DEGs were mainly associated with metabolism, transcription, ribosome and channel activity. We found 254,981 positive correlations
lncRNA-
mRNA pairs through correlation analysis. The detailed
lncRNA-
miRNA-
mRNA regulatory network included 11 lncRNAs, 35
miRNAs and 59 mRNAs. From the co-expression analyses, we identified 7 cis-regulated correlation pairs
lncRNA-
mRNA. Additionally, the qRT-PCR analysis confirmed our sequencing results. Our study constructed the
lncRNA-
mRNA-
miRNA regulation networks in
asthenozoospermia. Therefore, the study findings provide a set of pivotal lncRNAs for future investigation into the molecular mechanisms of
asthenozoospermia.