Temporal lobe epilepsy is the most common and refractory form of
epilepsy in adults. Gene expression within affected structures such as the hippocampus displays extensive dysregulation and is implicated as a central pathomechanism. Post-transcriptional mechanisms are increasingly recognized as determinants of the gene expression landscape, but key mechanisms remain unexplored. Here we show, for first time, that cytoplasmic
mRNA polyadenylation, one of the post-transcriptional mechanisms regulating gene expression, undergoes widespread reorganization in
temporal lobe epilepsy. In the hippocampus of mice subjected to
status epilepticus and
epilepsy, we report >25% of the transcriptome displays changes in their
poly(A) tail length, with deadenylation disproportionately affecting genes previously associated with
epilepsy. Suggesting cytoplasmic polyadenylation
element binding proteins (CPEBs) being one of the main contributors to
mRNA polyadenylation changes, transcripts targeted by CPEBs were particularly enriched among the gene pool undergoing
poly(A) tail alterations during
epilepsy. Transcripts bound by CPEB4 were over-represented among transcripts with
poly(A) tail alterations and
epilepsy-related genes and CPEB4 expression was found to be increased in mouse models of
seizures and resected hippocampi from patients with drug-refractory
temporal lobe epilepsy. Finally, supporting an adaptive function for CPEB4, deletion of Cpeb4 exacerbated seizure severity and neurodegeneration during
status epilepticus and the development of
epilepsy in mice. Together, these findings reveal an additional layer of gene expression regulation during
epilepsy and point to novel targets for seizure control and disease-modification in
epilepsy.