p53 and BLC2 Immunohistochemical Expression Across Molecular Subtypes in 1099 Early Breast Cancer Patients With Long-Term Follow-up: An Observational Study.

Abstract

INTRODUCTION:

p53 and antiapoptotic B-cell leukemia/lymphoma 2 (BLC2) have been proposed as prognostic markers for early breast cancer (BC), although their relationship with conventional parameters and patient prognosis, as well as their distribution within the molecular BC subtypes remains uncertain.

PATIENTS AND METHODS:

In this observational study, we analyzed the immunohistochemical expression of p53 and BLC2 in 1099 early BC patients surgically treated between 2000 and 2006 and followed for at least 5 years, also considering their association with pathologic factors and molecular subtypes, as well as their influence on disease-free survival.

RESULTS:

p53 and BLC2 are distributed differently across molecular subtypes (P < .0001); in particular, p53 positivity and BLC2 negativity seems to be associated with more aggressive conventional tumor phenotypes. Moreover, BLC2 negativity seems to be a significant discriminating factor for disease-free survival (P = .003) according to Kaplan-Meier analysis, while p53 seems to have no discriminating effect. Among patients with discordant p53/BLC2 phenotype, the combination p53+BLC2- seems to be associated with the worst outcomes (P = .007) and significantly influenced the clinical course of node-negative patients treated only with hormone therapy (P = .004).

CONCLUSION:

These two biomarkers, in addition to conventional pathologic factors and molecular subtype, could help define the risk and outcome of BC.

• Authors: Alessandra Fabi, Marcella Mottolese, Anna Di Benedetto, Francesca Sperati, Cristiana Ercolani, Simonetta Buglioni, Cecilia Nisticò, Gianluigi Ferretti, Patrizia Vici, Letizia Perracchio, Paola Malaguti, Michelangelo Russillo, Claudio Botti, Edoardo Pescarmona, Francesco Cognetti, Irene Terrenato
• Journal: Clinical breast cancer (Clin Breast Cancer) Vol. 20 Issue 6 Pg. e761-e770 (12 2020) ISSN: 1938-0666 [Electronic] United States
• PMID: 32580907 (Publication Type: Journal Article, Observational Study, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier Inc. All rights reserved.

Chemical References

• Antineoplastic Agents, Hormonal
• BCL2 protein, human
• Biomarkers, Tumor
• Proto-Oncogene Proteins c-bcl-2
• Receptors, Progesterone
• TP53 protein, human
• Tumor Suppressor Protein p53

Topics

• Adult
• Aged
• Aged, 80 and over
• Antineoplastic Agents, Hormonal (therapeutic use)
• Antineoplastic Combined Chemotherapy Protocols (therapeutic use)
• Biomarkers, Tumor (analysis, metabolism)
• Breast (pathology, surgery)
• Breast Neoplasms (diagnosis, mortality, pathology, therapy)
• Chemotherapy, Adjuvant (methods)
• Disease-Free Survival
• Female
• Follow-Up Studies
• Humans
• Immunohistochemistry
• Kaplan-Meier Estimate
• Mastectomy
• Middle Aged
• Neoplasm Recurrence, Local (epidemiology, pathology)
• Prognosis
• Proto-Oncogene Proteins c-bcl-2 (analysis, metabolism)
• Receptors, Progesterone
• Tumor Suppressor Protein p53 (analysis, metabolism)
• Young Adult