Abstract | BACKGROUND: 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG) is an active compound derived from Polygonum multiflorum Thunb., a Chinese Taoist herbal medicine, which exerts lipid lowering, anti- cancer, anti-aging, anti-inflammatory and hepatoprotective effects. However, its role in protecting hepatocytes under pre-diabetic condition remains unclear. METHODS: In this study, we developed prediabetic SD rats by feeding high-fat and high- sugar diet. The body weight, blood lipid, blood glucose, and fasting insulin (FINS) and insulin resistance index (HOMA-IR) were detected and calculated to assess the potential risk of prediabetes. HE and Oil Red O staining was used, and blood level of biochemical index was detected to observe the liver injury. The autophagic cell death-associated signaling proteins, and the potential signaling factors p-Akt/Akt and p-Erk/Erk were detected using western blot to explore the potential effects of TSG on pre-diabetic liver and the underlying mechanisms. RESULTS: The results showed that the body weight in TSG-treated group was significantly decreased vs. the model group. The blood glucose, the level of FINS and HOMA-IR, TC and TG were decreased in TSG-treated group as well. Furthermore, TSG treatment significantly ameliorated lipid droplet accumulation, enhanced liver anti-oxidative response which may be associated with an increased activity of SOD and GSH-Px, and a decrease of LDLC and MDA. The autophagic cell death-associated proteins, p-AMPK, ATG12, LC3 II, and Beclin 1 were up-regulated in the TSG-treated group, while the upstream signaling pathway, PI3K/Akt and Erk, were activated. CONCLUSIONS: TSG induced liver autophagic cell death to protect liver from prediabetic injury by activating PI3K/Akt and Erk.
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Authors | Xuanbin Wang, Jing Zeng, Xiao Wang, Ju Li, Jin Chen, Ning Wang, Miao Zhang, Yibin Feng, Huailan Guo |
Journal | BMC complementary medicine and therapies
(BMC Complement Med Ther)
Vol. 20
Issue 1
Pg. 177
(Jun 08 2020)
ISSN: 2662-7671 [Electronic] England |
PMID | 32513151
(Publication Type: Journal Article)
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Chemical References |
- Glucosides
- Stilbenes
- 2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside
- Proto-Oncogene Proteins c-akt
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Topics |
- Animals
- Autophagy
(drug effects)
- Fallopia multiflora
(chemistry)
- Glucosides
(pharmacology)
- Liver
(drug effects)
- MAP Kinase Signaling System
(drug effects)
- Male
- Molecular Structure
- Phosphatidylinositol 3-Kinases
(metabolism)
- Prediabetic State
(drug therapy)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Stilbenes
(pharmacology)
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