Abstract | BACKGROUND: METHODS: In the present study, the in vivo CPB-POCD models were established by using aged Sprague-Dawley (SD) male rats and the in vitro hypoxia/reoxygenation (H/R) models were inducted by using the primary hippocampus neuron (PHN) cells. RESULTS: The results showed that CPB impaired cognitive functions and induced hippocampus apoptosis in rat models, which were alleviated by pre-treating rats with low-dose sevoflurane. In addition, the phosphatidylinositol 3 kinase (PI3K)/ protein kinase B (AKT) signal pathway was inactivated in the hippocampus tissues of CPB-POCD rats, which were rescued by low-dose sevoflurane treatment. Of note, the PI3K/AKT inhibitor ( LY294002) abrogated the protective effects of low-dose sevoflurane on CPB-POCD rats. Consistently, the in vitro results showed that H/R treatment induced cell apoptosis and inhibited cell viability in PHN cells, which were attenuated by low-dose sevoflurane. Similarly, LY294002 abrogated the inhibiting effects of low-dose sevoflurane on H/R-induced PHN cell death. CONCLUSION: Taken together, low-dose sevoflurane attenuated CPB-induced POCD by inhibiting hippocampus apoptosis through activating PI3K/AKT signal pathway.
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Authors | Jianhua Qin, Qingjun Ma, Dongmei Ma |
Journal | Current neurovascular research
(Curr Neurovasc Res)
Vol. 17
Issue 3
Pg. 232-240
( 2020)
ISSN: 1875-5739 [Electronic] United Arab Emirates |
PMID | 32400333
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright© Bentham Science Publishers; For any queries, please email at [email protected]. |
Chemical References |
- Platelet Aggregation Inhibitors
- Sevoflurane
- Phosphatidylinositol 3-Kinase
- Proto-Oncogene Proteins c-akt
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Topics |
- Animals
- Apoptosis
(drug effects, physiology)
- Cardiopulmonary Bypass
(adverse effects)
- Dose-Response Relationship, Drug
- Hippocampus
(drug effects, metabolism)
- Male
- Phosphatidylinositol 3-Kinase
(metabolism)
- Platelet Aggregation Inhibitors
(administration & dosage)
- Postoperative Cognitive Complications
(metabolism, prevention & control)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Sevoflurane
(administration & dosage)
- Signal Transduction
(drug effects, physiology)
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