Endoplasmic reticulum (ER)
calcium homeostasis plays an essential role in cellular calcium signaling, intra-ER
protein chaperoning and maturation, as well as in the interaction of the ER with other organelles.
Calcium is accumulated in the ER by sarco/endoplasmic reticulum
calcium ATPases (SERCA
enzymes) that generate by active,
ATP-dependent transport, a several thousand-fold
calcium ion concentration gradient between the cytosol (low nanomolar) and the ER lumen (high micromolar). SERCA
enzymes are coded by three genes that by alternative splicing give rise to several
isoforms, which can display
isoform-specific
calcium transport characteristics. SERCA expression levels and
isoenzyme composition vary according to cell type, and this constitutes a mechanism whereby ER
calcium homeostasis is adapted to the signaling and metabolic needs of the cell, depending on its phenotype, its state of activation and differentiation. As reviewed here, in several normal epithelial cell types including bronchial, mammary, gastric, colonic and choroid plexus epithelium, as well as in mature cells of hematopoietic origin such as pumps are simultaneously expressed, whereas in corresponding
tumors and
leukemias SERCA3 expression is selectively down-regulated. SERCA3 expression is restored during the pharmacologically induced differentiation of various
cancer and
leukemia cell types. SERCA3 is a useful marker for the study of cell differentiation, and the loss of SERCA3 expression constitutes a previously unrecognized example of the remodeling of
calcium homeostasis in
tumors.