Abstract |
Non-small-cell lung cancer (NSCLC) is a complex disease which is influenced by multiple factors. Recent studies demonstrated that long non-coding RNA ( lncRNA) MIAT was involved in tumor metastasis. However, the underlying mechanism of MIAT in NSCLC remains largely unknown. In this study, MIAT, miR-139-5p and MMP2 expression were measured by quantitative reverse transcriptase PCR (QRT-PCR) or Western blotting, respectively, and we found the expression of MIAT and MMP2 were elevated, while miR-139-5p was decreased in NSCLC tissues and cell lines. Transwell assay showed MIAT and MMP2 functioned as an oncogene to induce cell migration and invasion in NSCLC, but miR-139-5p served as a tumor suppressor in NSCLC to inhibit cell migration and invasion. Besides that, in vivo experiments also indicated MIAT deletion inhibited tumor growth. The relationship between miR-139-5p and MIAT or MMP2 was then confirmed by Luciferase reporter assay, and the results showed that MIAT directly interacted with miR-139-5p and miR-139- 5p targetedly suppressed MMP2 in NSCLC cells. Furthermore, expression analysis showed that MIAT indirectly regulated MMP2 by sponging miR-139-5p. Finally, rescue assay suggested that miR-139-5p restoration reversed MIAT-overexpression-induced promotion on the migration and invasion of NSCLC cells. In conclusion, our results demonstrated that lncRNA MIAT modulated the migration and invasion of NSCLC by regulating miR-139-5p and MMP2.
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Authors | Fanye Zeng, Ning Yu, Yanyan Han, Julaiti Ainiwaer |
Journal | Journal of biosciences
(J Biosci)
Vol. 45
( 2020)
ISSN: 0973-7138 [Electronic] India |
PMID | 32345777
(Publication Type: Journal Article)
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Chemical References |
- MIRN139 microRNA, human
- Miat long non-coding RNA
- MicroRNAs
- RNA, Long Noncoding
- Matrix Metalloproteinase 2
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Topics |
- Animals
- Carcinoma, Non-Small-Cell Lung
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cells, Cultured
- Female
- Gene Expression Regulation, Neoplastic
- Gene Knockdown Techniques
- Humans
- Lung Neoplasms
(genetics, metabolism, pathology)
- Matrix Metalloproteinase 2
(metabolism)
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- MicroRNAs
(metabolism)
- Neoplasm Invasiveness
- RNA, Long Noncoding
(genetics, metabolism, physiology)
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