MicroRNA-186-5p serves as a diagnostic biomarker in atherosclerosis and regulates vascular smooth muscle cell proliferation and migration.

Abstract

OBJECTIVE:

MicroRNA dysregulation occurs in many human diseases, including atherosclerosis. Here, we examined the serum expression and clinical significance of miR-186-5p in patients with atherosclerosis, and explored its influence on vascular smooth muscle cell (VSMC) proliferation and migration.

METHODS:

Blood samples were collected from 104 patients with asymptomatic atherosclerosis and 80 healthy controls. Quantitative real-time PCR was applied to measure the miR-186-5p level. An ROC curve was established to assess the discriminatory ability of the serum miR-186-5p level for identifying atherosclerosis from controls. CCK-8 and Transwell assays were used to evaluate the impact of miR-186-5p on cell behaviors.

RESULTS:

Serum expression of miR-186-5p was significantly higher in atherosclerosis patients than in the control group. The serum miR-186-5p level showed a positive correlation with CIMT and could be used to distinguish atherosclerosis patients from healthy controls, with an area under the curve (AUC) score of 0.891. In VSMCs, overexpression of miR-186-5p significantly promoted cell proliferation and migration, while the opposite results were observed when miR-186-5p was downregulated.

CONCLUSION:

Overexpression of miR-186-5p has a certain diagnostic significance for atherosclerosis. Upregulation of miR-186-5p stimulates VSMC proliferation and migration. Therefore, it is a possible target for atherosclerosis interventions.

• Authors: Bin Sun, Qingtao Cao, Meng Meng, Xiaolong Wang
• Journal: Cellular & molecular biology letters (Cell Mol Biol Lett) Vol. 25 Pg. 27 ( 2020) ISSN: 1689-1392 [Electronic] England
• PMID: 32336973 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2020.

Chemical References

• Biomarkers
• MIRN186 microRNA, human
• MicroRNAs

Topics

• Adult
• Atherosclerosis (blood, genetics, pathology)
• Biomarkers (blood)
• Carotid Intima-Media Thickness
• Case-Control Studies
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Female
• Humans
• Male
• MicroRNAs (blood, genetics)
• Middle Aged
• Muscle, Smooth, Vascular (cytology, physiology)
• Sensitivity and Specificity