Objectives.
Hyperuricemia (HUA) is a disease caused by increased production of
uric acid (UA) or reduced excretion of UA in the body. Results of an epidemiological survey show that 60% of patients with HUA have
hyperlipidemia (HPA). Dendrobium officinalis (DOF) six nostrum (DOS) is based on the theory of
traditional Chinese medicine for the transformation of the traditional Chinese nostrum Si Miao Wan. In this article, we aim to discuss the efficacy and mechanism of DOS in reducing UA and regulating lipid metabolism. The rat model of HUA with HPA was induced by
potassium oxonate (PO) combined with high-fat sorghum feed. We monitored the serum UA and blood
lipids. Liver
xanthine oxidase (XOD),
adenosine deaminase (ADA),
lipoprotein lipase (LPL), and
fatty acid-binding protein (FABP1) activities were measured by
enzyme-linked
immunosorbent assay (ELISA) after the last administration of DOS. We performed a histopathological examination of rat kidney and intestine. Immunohistochemistry (IHC) was used to detect the expression of renal inflammatory
proteins NLRP3 / Caspase-1 and intestinal inflammatory
proteins TLR4 / NLRP3. We used western blot for measurement of liver
hypoxanthine-
guanine phosphoribosyl
transferase (HPRT1)
protein expression and renal PDZ domain
protein kidney 1 (PDZK1)
protein expression. DOS administration significantly reduced serum UA, total
cholesterol (TC), and
low-density lipoprotein cholesterol (
LDL-c) level, and improved
liver steatosis in the model rat. At the same time, DOS treatment effectively inhibited liver XOD and ADA, increased the level of liver HPRT1, and reduced the production of UA. Additional studies had shown that DOS can restore normal UA excretion function in the intestine and kidney and regulated liver
lipids metabolism. IHC and histopathological sections showed that DOS reduced the level of kidney, intestinal inflammatory body (NLRP3,
Caspase-1, and TLR4), improved
inflammation of the kidney and intestinal tract in rats. DOS is a promising
drug that can effectively reduce serum UA and
lipid level in the model rat. The mechanism of action may be related to inhibition of UA production, promotion of UA excretion, regulation of
lipids metabolism, and anti-inflammatory response.