Quaternary ammonium silane, calcium and phosphorus-loaded PLGA submicron particles against Enterococcus faecalis infection of teeth: An in vitro and in vivo study.

Abstract

Refractory root canal infection of human teeth is the primary cause of dental treatment failure. Enterococcus faecalis is the major cause of refractory root canal infection. In the present study, poly(D,L-lactic-co-glycolide) (PLGA) submicron particles were used as carriers to deliver an antimicrobial quaternary ammonium silane (code-named K21) as well as calcium and phosphorus elements. The release profiles, antibacterial ability against E. faecalis, extent of infiltration into dentinal tubules, biocompatibility and in vitro mineralization potential of the particles were investigated. In addition, the antimicrobial effects of the particles against E. faecalis infection were evaluated in vivo in the teeth of beagle dogs. The encapsulated components were released from the PLGA particles in a sustained-release manner. The particles also displayed good biocompatibility, in vitro mineralization ability and antibacterial activity against E. faecalis. The particles could be driven into dentinal tubules of dentin slices by ultrasonic activation and inhibited E. faecalis colonization. In the root canals of beagle dogs, PLGA submicron particles loaded with K21, calcium and phosphorus demonstrated strong preventive effects against E. faecalis infection. The system may be developed into a new intracanal disinfectant for root canal treatment.

• Authors: Wei Fan, Yanyun Li, Qing Sun, Franklin R Tay, Bing Fan
• Journal: Materials science & engineering. C, Materials for biological applications (Mater Sci Eng C Mater Biol Appl) Vol. 111 Pg. 110856 (Jun 2020) ISSN: 1873-0191 [Electronic] Netherlands
• PMID: 32279748 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Quaternary Ammonium Compounds
• Silanes
• Polylactic Acid-Polyglycolic Acid Copolymer
• Phosphorus
• Tartrate-Resistant Acid Phosphatase
• Calcium

Topics

• Animals
• Anti-Bacterial Agents (pharmacology)
• Calcium (pharmacology)
• Cell Line
• Dogs
• Enterococcus faecalis (drug effects)
• Female
• Hydrogen-Ion Concentration
• Microbial Sensitivity Tests
• Particle Size
• Phosphorus (pharmacology)
• Polylactic Acid-Polyglycolic Acid Copolymer (chemistry)
• Quaternary Ammonium Compounds (chemistry, pharmacology)
• Silanes (pharmacology)
• Static Electricity
• Tartrate-Resistant Acid Phosphatase (metabolism)
• Tooth (diagnostic imaging, microbiology, pathology)