MiR-429-5p attenuates the migration and invasion of malignant melanoma by targeting LIMK1.

Abstract

OBJECTIVE:

To investigate the potential effects of microRNA-429-5p (miR-429-5p) on the development of malignant melanoma (MM) and the relevant mechanism.

PATIENTS AND METHODS:

Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the differential expression of miR-429-5p in MM tissues. The relationship between miR-429-5p expression and clinical pathological data of MM patients was analyzed. LIM kinase 1 (LIMK1) was verified as a downstream target of miR-429-5p by online prediction software, and the interaction between LIMK1 and miR-429-5p was verified by Dual-Luciferase reporter assay.

RESULTS:

Compared with normal skin tissues, miR-429-5p was downregulated in MM tissues. MiR-429-5p expression was correlated with tumor size and stage of MM. Upregulation of miR-429-5p significantly inhibited protein expression of LIMK1 and reduced migration and invasion ability of MM cells. LIMK1 was involved in MM progression regulated by miR-429-5p.

CONCLUSIONS:

MiR-429-5p attenuates migration and invasion in MM by targeting LIMK1. Hence, miR-429-5p/LIMK1 axis might be a potential therapeutic target for the treatment of MM.

• Authors: H Sheng, Y-H Guo, D-S Cao, X-J Li, Y Zhao, H Ding, Y-L Zhang, Z-H Chen
• Journal: European review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 24 Issue 5 Pg. 2625-2631 (03 2020) ISSN: 2284-0729 [Electronic] Italy
• PMID: 32196612 (Publication Type: Journal Article)

Chemical References

• MIRN429 microRNA, human
• MicroRNAs
• LIMK1 protein, human
• Lim Kinases

Topics

• Cell Movement
• Cells, Cultured
• Female
• Humans
• Lim Kinases (genetics, metabolism)
• Male
• Melanoma (metabolism, pathology)
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Up-Regulation