Abstract | OBJECTIVES: Microvesicles (MVs) derived from human Wharton's jelly mesenchymal stem cells (MSC-MVs) were demonstrated to ameliorate acute lung injury (ALI). We have previously found that MSC-MV-transferred hepatocyte growth factor was partly involved in their therapeutic effects. Since MSC-MVs also contained a substantial quantity of miR-100, which plays an important role in lung cancer and injury, we speculated that miR-100 might similarly account for a part of the therapeutic effects of MSC-MVs. METHODS: MSCs were transfected with miR-100 inhibitor to downregulate miR-100 in MSC-MVs. A rat model of ALI and cell injury in rat type II alveolar epithelial cell line (L2) was induced by bleomycin (BLM). A co-culture model of alveolar epithelial cells and MSC-MVs was utilized to examine the therapeutic role of MSC-MVs and mechanism. RESULTS: MSC-MV treatment attenuated BLM-induced apoptosis and inflammation in BLM-treated L2 cells and ameliorated BLM-induced lung apoptosis, inflammation, and fibrosis in BLM-induced ALI rats. The beneficial effect of MSC-MVs was partly eliminated when miR-100 was knocked down in MSCs. Moreover, MSC-MV-transferred miR-100 mediated the therapeutic effect of MSC-MVs in ALI through enhancing autophagy by targeting mTOR. CONCLUSION: MSC-MVs enhance autophagy and ameliorate ALI partially via delivery of miR-100.
|
Authors | Wen-Xia Chen, Jun Zhou, Sha-Sha Zhou, Yu-Dan Zhang, Tong-Yu Ji, Xiao-Li Zhang, Shu-Min Wang, Tao Du, De-Gang Ding |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 11
Issue 1
Pg. 113
(03 13 2020)
ISSN: 1757-6512 [Electronic] England |
PMID | 32169098
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- MIRN100 microRNA, human
- MIRN100 microRNA, rat
- MicroRNAs
|
Topics |
- Acute Lung Injury
(chemically induced, genetics, therapy)
- Animals
- Autophagy
- Humans
- Mesenchymal Stem Cells
- MicroRNAs
(genetics)
- Rats
- Wharton Jelly
|