Moyamoya disease patient mutations in the RING domain of RNF213 reduce its ubiquitin ligase activity and enhance NFκB activation and apoptosis in an AAA+ domain-dependent manner.

Abstract

Moyamoya disease (MMD) is a cerebrovascular disease characterized by progressive occlusion of the internal carotid arteries. Genetic studies originally identified RNF213 as an MMD susceptibility gene that encodes a large 591 kDa protein with a functional RING domain and dual AAA+ ATPase domains. As the functions of RNF213 and its relationship to MMD onset are unknown, we set out to characterize the ubiquitin ligase activity of RNF213, and the effects of MMD patient mutations on these activities and on other cellular processes. In vitro ubiquitination assays, using the RNF213 RING domain, identified Ubc13/Uev1A as a key ubiquitin conjugating enzyme that together generate K63-linked polyubiquitin chains. However, nearly all MMD patient mutations in the RING domain greatly reduced this activity. When full-length proteins were overexpressed in HEK293T cells, patient mutations that abolished the ubiquitin ligase activities conversely enhanced nuclear factor κB (NFκB) activation and induced apoptosis accompanied with Caspase-3 activation. These induced activities were dependent on the RNF213 AAA+ domain. Our results suggest that the NFκB- and apoptosis-inducing functions of RNF213 may be negatively regulated by its ubiquitin ligase activity and that disruption of this regulation could contribute towards MMD onset.

• Authors: Midori Takeda, Tohru Tezuka, Minsoo Kim, Jungmi Choi, Yuki Oichi, Hatasu Kobayashi, Kouji H Harada, Tsunehiro Mizushima, Shigeru Taketani, Akio Koizumi, Shohab Youssefian
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 525 Issue 3 Pg. 668-674 (05 07 2020) ISSN: 1090-2104 [Electronic] United States
• PMID: 32139119 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Mutant Proteins
• NF-kappa B
• Transcription Factors
• Polyubiquitin
• UBE2N protein, human
• UBE2V1 protein, human
• Ubiquitin-Conjugating Enzymes
• RNF213 protein, human
• Ubiquitin-Protein Ligases
• Adenosine Triphosphatases
• Lysine

Topics

• AAA Domain
• Adenosine Triphosphatases (chemistry, genetics)
• Amino Acid Sequence
• Apoptosis
• HEK293 Cells
• Humans
• Lysine (metabolism)
• Moyamoya Disease (genetics)
• Mutant Proteins (chemistry, metabolism)
• Mutation (genetics)
• NF-kappa B (metabolism)
• Polyubiquitin (metabolism)
• RING Finger Domains
• Transcription Factors (metabolism)
• Ubiquitin-Conjugating Enzymes (metabolism)
• Ubiquitin-Protein Ligases (chemistry, genetics)