Quercetin and Its Mixture Increase the Stress Resistance of Caenorhabditis elegans to UV-B.

Abstract

Ultraviolet B (UV-B, 280-320 nm) radiation causes complex molecular reactions in cells, including DNA damage, oxidative stress, and apoptosis. This study designed a mixture consisting of quercetin, luteolin and lycopene and used Caenorhabditis elegans as a model to study the resistance of these natural chemicals to UV-B. Specifically, we have confirmed that quercetin and its mixture can increase the resistance of Caenorhabditis elegans to UV-B through lifespan test, reactive oxygen species level assay, germ cell apoptosis test, embryonic lethal test and RT-qPCR experiments. The results show that quercetin and its mixture prolonged the lifespan of UV-B-irradiated Caenorhabditis elegans and reduced abnormal levels of reactive oxygen species, embryo death, and apoptosis induced by UV-B. The protective effect of quercetin and its mixture may be attributed to its down-regulation of HUS-1, CEP-1, EGL-1 and CED-13. Therefore, the results of this research could help the development of UV-B radiation protection agents.

• Authors: Shi-Ming Li, Dan Liu, Yi-Lin Liu, Bin Liu, Xing-Huang Chen
• Journal: International journal of environmental research and public health (Int J Environ Res Public Health) Vol. 17 Issue 5 (02 29 2020) ISSN: 1660-4601 [Electronic] Switzerland
• PMID: 32121354 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Caenorhabditis elegans Proteins
• Quercetin

Topics

• Animals
• Antioxidants (pharmacology)
• Apoptosis
• Caenorhabditis elegans (physiology)
• Caenorhabditis elegans Proteins
• DNA Damage
• Germ Cells
• Oxidative Stress
• Quercetin (pharmacology)
• Stress, Physiological (drug effects)