Activation of Estrogen Receptor G Protein-Coupled Receptor 30 Enhances Cholesterol Cholelithogenesis in Female Mice.

Abstract

BACKGROUND AND AIMS:

Estrogen is an important risk factor for cholesterol gallstone disease because women are twice as likely as men to form gallstones. The classical estrogen receptor α (ERα), but not ERβ, in the liver plays a critical role in the formation of estrogen-induced gallstones in female mice. The molecular mechanisms underlying the lithogenic effect of estrogen on gallstone formation have become more complicated with the identification of G protein-coupled receptor 30 (GPR30), an estrogen receptor.

APPROACH AND RESULTS:

We investigated the biliary and gallstone phenotypes in ovariectomized female GPR30-/- , ERα-/- , and wild-type mice injected intramuscularly with the potent GPR30-selective agonist G-1 at 0 or 1 μg/day and fed a lithogenic diet for 8 weeks. The activation of GPR30 by G-1 enhanced cholelithogenesis by suppressing expression of cholesterol 7α-hydroxylase, the rate-limiting enzyme for the classical pathway of bile salt synthesis. These metabolic abnormalities led to an increase in biliary cholesterol concentrations in company with hepatic hyposecretion of biliary bile salts, thereby inducing cholesterol-supersaturated gallbladder bile and accelerating cholesterol crystallization. G-1 also impairs gallbladder emptying, leading to sluggish gallbladder motility and promoting the development of biliary sludge in the early stage of gallstone formation. The prevalence rates of gallstones were 80% in wild-type and ERα-/- mice treated with G-1 compared to 10% in wild-type mice receiving no G-1. However, no gallstones were formed in GPR30-/- mice treated with G-1.

CONCLUSIONS:

GPR30 produces additional lithogenic actions, working independently of ERα, to increase susceptible to gallstone formation in female mice; both GPR30 and ERα are potential therapeutic targets for cholesterol gallstone disease, particularly in women and patients exposed to high levels of estrogen.

• Authors: Helen H Wang, Ornella de Bari, Christopher K Arnatt, Min Liu, Piero Portincasa, David Q-H Wang
• Journal: Hepatology (Baltimore, Md.) (Hepatology) Vol. 72 Issue 6 Pg. 2077-2089 (12 2020) ISSN: 1527-3350 [Electronic] United States
• PMID: 32112420 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © 2020 by the American Association for the Study of Liver Diseases.

Chemical References

• Bile Acids and Salts
• Esr1 protein, mouse
• Estrogen Receptor alpha
• Estrogens
• G-1 ethanone compound
• GPER1 protein, mouse
• Quinolines
• Receptors, Estrogen
• Receptors, G-Protein-Coupled
• Cholesterol

Topics

• Animals
• Bile (drug effects, metabolism)
• Bile Acids and Salts (biosynthesis)
• Cholesterol (metabolism)
• Disease Models, Animal
• Estrogen Receptor alpha (genetics, metabolism)
• Estrogens (metabolism)
• Female
• Gallbladder (pathology)
• Gallstones (pathology)
• Humans
• Lipid Metabolism (drug effects)
• Liver (metabolism)
• Male
• Mice
• Mice, Knockout
• Ovariectomy
• Quinolines (administration & dosage)
• Receptors, Estrogen (genetics, metabolism)
• Receptors, G-Protein-Coupled (agonists, genetics, metabolism)
• Sex Factors