KIF15 Expression in Tumor-associated Monocytes Is a Prognostic Biomarker in Hepatocellular Carcinoma.

Abstract

BACKGROUND/AIM:

Kinesin family member 15 (KIF15) participates in the transport of macromolecules in essential cellular processes. In this study we evaluated the clinical relevance of KIF15 expression in hepatocellular carcinoma (HCC).

MATERIALS AND METHODS:

Association between KIF15 expression and clinical outcomes in HCC patients was analyzed using three independent cohorts. Localization of KIF15 expression was assessed by immunohistochemical analysis. Co-culture experiments were performed using healthy donor peripheral blood mononuclear cells (PBMC) and HCC cell lines.

RESULTS:

Immunohistochemical analysis showed that KIF15 was mainly expressed in inflammatory monocytes around cancer cells. Multivariate analysis indicated high KIF15 expression was an independent poor prognostic factor for survival. HCC cells with high expression of minichromosome maintenance protein 2 (MCM2) were located close to KIF15-expressing inflammatory monocytes. The proliferation ability of HCC cells was increased by co-culture with PBMC.

CONCLUSION:

High KIF15 expression in inflammatory monocytes in tumor tissues may serve as a prognostic marker for poor outcome in HCC.

• Authors: Akihiro Kitagawa, Takaaki Masuda, Junichi Takahashi, Taro Tobo, Miwa Noda, Yosuke Kuroda, Qingjiang Hu, Yuta Kouyama, Yuta Kobayashi, Shotaro Kuramitsu, Kuniaki Sato, Atsushi Fujii, Yukihiro Yoshikawa, Hiroaki Wakiyama, Dai Shimizu, Yusuke Tsuruda, Hidetoshi Eguchi, Yuichiro Doki, Masaki Mori, Koshi Mimori
• Journal: Cancer genomics & proteomics (Cancer Genomics Proteomics) 2020 Mar-Apr Vol. 17 Issue 2 Pg. 141-149 ISSN: 1790-6245 [Electronic] Greece
• PMID: 32108036 (Publication Type: Journal Article)
• Copyright: Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• KIF15 protein, human
• Kinesins

Topics

• Aged
• Carcinoma, Hepatocellular (genetics, mortality)
• Cell Line, Tumor
• Female
• Humans
• Kinesins (metabolism)
• Liver Neoplasms (genetics, mortality)
• Male
• Middle Aged
• Monocytes (metabolism)
• Prognosis
• Survival Rate