Cardiac protection of Bauhinia championii against reperfusion injury.

Abstract

This study aims to investigate the protective effects of the Bauhinia championii (BC) against ischemia/reperfusion (I/R)-induced injury in an isolated heart model. Langendorff-perfused C57BL/6JNarl mice hearts were performed with 30 minutes ischemia and 60 minutes reperfusion by left anterior descending artery ligation. Before reperfusion, boiling water extracts of BC (10 mg/L) was pretreated for 15 minutes. During reperfusion, BC significantly decreased the occurrence of ventricular arrhythmias by lead II electrocardiogram (ECG). Electrophysiological effect of BC was further determined in isolated ventricular myocytes by whole-cell patch clamp technique. The underlying mechanism may result from its Na+ channel blocking activity characterized with reduced rise slope of action potential and Na+ current density. Moreover, BC dramatically reduced I/R-caused infarct size, which was accessed by 2,3,5-triphenyltetrazolium chloride (TTC) assay. Since BC decreased I/R-induced myoglobin release and oxidation of Ca2+ -calmodulin-dependent protein kinase, inhibition of myocardial necroptosis may account for the protective effects of BC on myocytes lose. This study indicated that BC may prevent I/R induced ventricular arrhythmias and myocyte death by blocking Na+ channels and decreasing necroptosis, respectively. Since most of the available antiarrhythmic remedies have unwanted adverse actions, BC could be a novel candidate for the treatment of myocardial infarction and ventricular arrhythmia.

• Authors: Yun-Fang Chen, Wei-Yu Chen, Ching-Hu Chung, Chao-Lin Kuo, An-Sheng Lee
• Journal: Environmental toxicology (Environ Toxicol) Vol. 35 Issue 7 Pg. 774-782 (Jul 2020) ISSN: 1522-7278 [Electronic] United States
• PMID: 32061153 (Publication Type: Journal Article)
• Copyright: © 2020 Wiley Periodicals, Inc.

Chemical References

• Plant Extracts
• Sodium Channel Blockers
• Sodium Channels

Topics

• Animals
• Bauhinia (chemistry)
• Electrocardiography
• Heart (drug effects)
• In Vitro Techniques
• Male
• Membrane Potentials (drug effects)
• Mice
• Mice, Inbred C57BL
• Myocardial Infarction (metabolism, pathology, prevention & control)
• Myocardial Reperfusion Injury (metabolism, pathology, prevention & control)
• Myocardium (metabolism, pathology)
• Myocytes, Cardiac (metabolism, pathology)
• Necroptosis (drug effects)
• Patch-Clamp Techniques
• Plant Components, Aerial (chemistry)
• Plant Extracts (isolation & purification, pharmacology)
• Sodium Channel Blockers (isolation & purification, pharmacology)
• Sodium Channels (metabolism)