Abstract |
Transforming growth factor-β, a cell secretion factor of the TGF-β superfamily, is involved in the regulation of cell proliferation, differentiation, cytoskeleton formation, migration, invasion and other biological behaviors. Autophagy and mitophagy play an important role in tumor progression by regulating self-digestion, and degradation and reuse of cells and mitochondria. In this study, changes in autophagy and mitophagy processes in ovarian cancer cells under TGF-β1 treatment were detected via Western blot and immunofluorescence, as well as the role of fucosylation modification. Changes in mitochondrial membrane potential in response to TGF-β1 and fucosylation were detected via immunofluorescence. The effects of TGF-β1 and its fucosylation on autophagic flux were further determined by transient transfection of cells with Ad-mRFP-GFP-LC3 adenovirus. TGF-β1 clearly promoted autophagy and mitophagy in ovarian cancer cells. TGF-β1 fucosylation stimulated these regulatory effects on ovarian cancer cells via modulation of PI3K/Akt and Ras-Raf-MEK-ERK pathways through TAK1. Our collective data support the physiological significance of TGF-β1 and provide a novel direction for targeted therapy for ovarian cancer.
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Authors | Shan Jin, Jian Gao, Yue Qi, Yingying Hao, Xiao Li, Qing Liu, Juanjuan Liu, Dawo Liu, Liancheng Zhu, Bei Lin |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 524
Issue 4
Pg. 970-976
(04 16 2020)
ISSN: 1090-2104 [Electronic] United States |
PMID | 32059847
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 Elsevier Inc. All rights reserved. |
Chemical References |
- TGFB1 protein, human
- Transforming Growth Factor beta1
- Fucose
- Proto-Oncogene Proteins c-akt
- raf Kinases
- ras Proteins
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Topics |
- Autophagy
- Cell Line, Tumor
- Female
- Fucose
(metabolism)
- Glycosylation
- Humans
- MAP Kinase Signaling System
- Mitophagy
- Ovarian Neoplasms
(metabolism, pathology)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Signal Transduction
- Transforming Growth Factor beta1
(metabolism)
- raf Kinases
(metabolism)
- ras Proteins
(metabolism)
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