Staphylococcus aureus Fibronectin Binding Protein A Mediates Biofilm Development and Infection.

Abstract

Implanted medical device-associated infections pose significant health risks, as they are often the result of bacterial biofilm formation. Staphylococcus aureus is a leading cause of biofilm-associated infections which persist due to mechanisms of device surface adhesion, biofilm accumulation, and reprogramming of host innate immune responses. We found that the S. aureus fibronectin binding protein A (FnBPA) is required for normal biofilm development in mammalian serum and that the SaeRS two-component system is required for functional FnBPA activity in serum. Furthermore, serum-developed biofilms deficient in FnBPA were more susceptible to macrophage invasion, and in a model of biofilm-associated implant infection, we found that FnBPA is crucial for the establishment of infection. Together, these findings show that S. aureus FnBPA plays an important role in physical biofilm development and represents a potential therapeutic target for the prevention and treatment of device-associated infections.

• Authors: Casey M Gries, Trevor Biddle, Jeffrey L Bose, Tammy Kielian, David D Lo
• Journal: Infection and immunity (Infect Immun) Vol. 88 Issue 5 (04 20 2020) ISSN: 1098-5522 [Electronic] United States
• PMID: 32041788 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2020 American Society for Microbiology.

Chemical References

• Adhesins, Bacterial
• Bacterial Proteins
• fibronectin-binding proteins, bacterial

Topics

• Adhesins, Bacterial (metabolism)
• Animals
• Bacterial Adhesion (physiology)
• Bacterial Proteins (metabolism)
• Biofilms (growth & development)
• Humans
• Mice
• Mice, Inbred C57BL
• Protein Binding (physiology)
• Staphylococcal Infections (metabolism, microbiology)
• Staphylococcus aureus (metabolism, pathogenicity)