Abstract | OBJECTIVE: MATERIALS AND METHODS: The expression of HAL and TWIST1 was detected by qRT-PCR. CCK8 assay, wound healing assay, transwell assay and flow cytometry were used to detect the HAL function on proliferation, migration, invasion and apoptosis in SOC cells. Western blot was used to calculate protein level of Vimentin, N-cadherin and E-cadherin. The effect of HAL on tumorigenesis of SOC was confirmed by xenograft nude mice model. RESULTS: HAL was significantly decreased in SOC tissues and cells. Overexpression of HAL inhibited the proliferation, migration and invasion of SKOV3 cells, but promoted apoptosis. Furthermore, overexpression of HAL decreased the mRNA and protein levels of TWIST1 via a binding between HAL and TWIST1. Forced expression of TWIST1 reversed the inhibitory role of HAL on SOC cells' migration and invasion. The in vivo tumor growth assay showed that HAL suppressed SOC tumorigenesis with inhibiting EMT pathway. CONCLUSIONS: Our research emphasized HAL acting as a tumor-inhibiting gene by regulating EMT signaling pathway, thus providing some novel experimental basis for clinical treatment of SOC.
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Authors | Ke Wu, Lei Li, Lin Li, Dong Wang |
Journal | Bioscience reports
(Biosci Rep)
Vol. 40
Issue 3
(03 27 2020)
ISSN: 1573-4935 [Electronic] England |
PMID | 32039453
(Publication Type: Journal Article, Retracted Publication)
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Copyright | © 2020 The Author(s). |
Chemical References |
- Nuclear Proteins
- RNA, Long Noncoding
- TWIST1 protein, human
- Twist-Related Protein 1
- Histidine Ammonia-Lyase
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Topics |
- Animals
- Apoptosis
(physiology)
- Carcinoma, Ovarian Epithelial
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
(physiology)
- Cell Proliferation
(physiology)
- Cystadenocarcinoma, Serous
(genetics, metabolism, pathology)
- Epithelial-Mesenchymal Transition
- Female
- Histidine Ammonia-Lyase
(genetics)
- Humans
- Mice
- Mice, Nude
- Nuclear Proteins
(genetics, metabolism)
- Ovarian Neoplasms
(genetics, metabolism, pathology)
- RNA, Long Noncoding
(genetics)
- Signal Transduction
- Twist-Related Protein 1
(genetics, metabolism)
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