Chronic liver
inflammation leads to
fibrosis and
cirrhosis and is associated with an accumulation of intrahepatic TNFα-secreting CD206+ macrophages, which may participate in maintaining chronic
liver disease in a
GM-CSF-dependent manner. We aimed to elucidate the exact role of
GM-CSF in the development and progression of chronic
liver disease.
METHODS: Liver immunohistochemistry and serum quantification were performed in patients with viral and non-viral-related
liver disease to compare CD206+ monocyte/macrophages,
fibrosis and
GM-CSF. This was followed by functional validations in vitro and in vivo in humanised mice.
RESULTS: Using multiplex immunofluorescence and histo-cytometry, we show that highly fibrotic livers had a greater density of CD206+ macrophages that produced more TNFα and
GM-CSF in the non-tumour liver regions of patients with
hepatocellular carcinoma (n = 47), independent of aetiology. In addition, the absolute number of CD206+ macrophages strongly correlated with the absolute number of
GM-CSF-producing macrophages. In non-HCC chronic HCV+ patients (n = 40), circulating
GM-CSF levels were also increased in proportion to the degree of
liver fibrosis and serum viral titres. We then demonstrated in vitro that monocytes converted to TNFα-producing CD206+ macrophage-like cells in response to bacterial products (
lipopolysaccharide) in a
GM-CSF-dependent manner, confirming the in vivo normalisation of serum
GM-CSF concentration following oral
antibiotic treatment observed in HBV-infected humanised mice. Finally, anti-
GM-CSF neutralising antibody treatment reduced intrahepatic CD206+ macrophage accumulation and abolished
liver fibrosis in HBV-infected humanised mice.
CONCLUSIONS: LAY SUMMARY: