Hypertension, which is known as a silent killer, is the second leading cause of
kidney failure worldwide. Elevated blood pressure causes approximately 7.6 million deaths, which account for ~13.5% of the total deaths and will continue to rise.
High blood pressure is the prime risk factor associated with complications in major organs, including the heart, brain and kidney.
High blood pressure accelerates oxidative stress and thereby causes organ dysfunction through the production of
reactive oxygen species. In this study, we investigated the renal-protective effects of the bioactive
peptide IF from
alcalase potato
protein hydrolysate in spontaneously hypertensive rat kidney. Sixteen-week-old spontaneously hypertensive rats were divided into three groups (n = 6), and Sixteen-week-old Wistar Kyoto rats (n = 6) served as the control group. The rats were administered IF and
captopril via oral gavage for 8 weeks and then sacrificed, and their kidneys were harvested. The kidney sections from the rats treated with IF showed restoration of the structure of the glomerulus and Bowman's capsule. The expression levels of Nrf2-mediated
antioxidants were also increased, as confirmed by
4-hydroxynonenal immunohistochemical staining. The TUNEL assay revealed a significant reduction in the number of apoptotic cells in the IF-treated groups, which was consistent with the western blot results. Thus, the bioactive
peptide IF exerts potential protective effects against
hypertension-associated ROS-mediated renal damage via the Nrf2-dependent
antioxidant pathway along the DJ-1 and AKT axes. Hence, we speculate that IF might have promising
therapeutic effects on renal damage associated with
hypertension.