[Dihydroartemisinin Induces AML cell Apoptosis by Inhibition of PTEN/AKT pathway].

Abstract

OBJECTIVE:

To study the effects of dihydroartemisinin (DHA) on the proliferation and apoptosis of acute myeloid leukemia (AML) cells.

METHODS:

The effects of DHA on the proliferation of acute myeloid leukemia cells and the inhibitory effect of Z-VAD-FMK on the DHA-induced cell apoptosis were detected by CCK-8 assay. The expression level of cleaved-caspased 3 was detected by indirect immunofluorescence. Western blot was used to quantify the protein expression of PTEN, p-Akt, AKT, β-actin, and the apoptosis-associated proteins, such as C-PARP, Cleaved-caspase3 and Caspase3 respectively.

RESULTS:

DHA induced the AML cell apoptosis with concentration-dependent manner (rKasumi-1=-0.959, rKG-1=-0.956). The DHA could induce the accumulation of cleaved-caspase 3 and C-PARP in AML cells, activate PTEN gene and inhibited Akt phosphorylation. Apoptosis inhibitor Z-VAD-FMK could partially restored the activity of DHA-inhibited cell proliferation.

CONCLUSION:

Dihydroartemisinin induces AML cell apoptosis by inhibition of PTEN/AKT pathway. Dihydroartemisinin is expected to be a safe and effective drug for treatment of acute myeloid leukemia.

• Authors: Yi-Han An, Jing DU, Zhi-Wen Hou, Wei-Dong Sun, Yi Zhou, Xing-Xing Yu, Xin Wang, Ying Wang, Xiang-Min Tong
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 28 Issue 1 Pg. 88-92 (Feb 2020) ISSN: 1009-2137 [Print] China
• PMID: 32027258 (Publication Type: Journal Article)

Chemical References

• Artemisinins
• artenimol
• Proto-Oncogene Proteins c-akt
• PTEN Phosphohydrolase
• PTEN protein, human

Topics

• Apoptosis
• Artemisinins
• Cell Line, Tumor
• Cell Proliferation
• Humans
• Leukemia, Myeloid, Acute
• PTEN Phosphohydrolase
• Proto-Oncogene Proteins c-akt
• Signal Transduction