Role of lncRNA-ATB in ovarian cancer and its mechanisms of action.

Abstract

This study aimed to elucidate the role of long non-coding RNA activated by transforming growth factor-β (lncRNA-ATB) in ovarian cancer and its underlying mechanisms of action. Expression levels of lncRNA-ATB in ovarian cancer cell line SKOV3 and in a healthy human ovarian cell line were compared using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results indicated that lncRNA-ATB was expressed at significantly higher levels in SKOV3 cells compared with the healthy cell line. After downregulation of lncRNA-ATB expression in SKOV3 cells using lncRNA-ATB-short hairpin RNA, cell proliferation, apoptosis, invasion and migration were assessed using Cell counting kit-8, Live Dead staining, Transwell assay and wound healing assay, respectively. RT-qPCR and western blotting were used to quantify the expression of signal transducer and activator of transcription 3 (STAT3), phosphorylated (p)-STAT3, and the additional epithelial to mesenchymal transition (EMT)-related proteins E-cadherin and vimentin in SKOV3 cells. LncRNA-ATB downregulation significantly reduced SKOV3 cell proliferation, invasion and migration, promoted apoptosis, decreased the expression of p-STAT3 and vimentin, and increased E-cadherin expression. Taken together, these results suggest that lncRNA-ATB downregulation can inhibit ovarian cancer cell proliferation, invasion and migration, and promote cell apoptosis. Lnc-RNA-ATB may therefore be a new target for ovarian cancer treatment.

• Authors: Donglan Yuan, Xiaofang Zhang, Yinling Zhao, Hua Qian, Hezhu Wang, Cuiqin He, Xia Liu, Ting Guo, Mei Lin, Hong Yu, Jun Ye
• Journal: Experimental and therapeutic medicine (Exp Ther Med) Vol. 19 Issue 2 Pg. 965-971 (Feb 2020) ISSN: 1792-0981 [Print] Greece
• PMID: 32010258 (Publication Type: Journal Article)
• Copyright: Copyright: © Yuan et al.