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Non-thermal atmospheric pressure plasma induces epigenetic modifications that activate the expression of various cytokines and growth factors in human mesoderm-derived stem cells.

Abstract
Non-thermal atmospheric pressure plasma (NTAPP) has been reported to induce wound healing, activation of immune cells, and proliferation of mesoderm-derived adult stem cells in human. However, the mechanism by which NTAPP activates these physiological effects is poorly understood. Here, we examined whole genome expression profiles of adipose tissue-derived stem cells (ASCs), the proliferation of which is induced by NTAPP. NTAPP upregulated the expression of genes for cytokine and growth factor, but downregulated genes in apoptotic pathways. When ASCs were treated with NTAPP in the presence of a nitric oxide (NO) scavenger, the expression of various cytokines and growth factors decreased, suggesting that NO is primarily responsible for the enhanced cytokine and growth factor expression induced by NTAPP. Increased histone deacetyl transferase 1 (HDAC1) and decreased acetylated histone 3 were detected in NTAPP-treated ASCs. Similarly, ASCs pre-treated with HDAC, DNA methylation, or histone methylation inhibitors had reduced expression of cytokines and growth factors after NTAPP treatment. Taken together, these results strongly suggest that NTAPP induces epigenetic modifications that activate the expression of cytokines and growth factors, explaining how NTAPP acts as an efficient tool in regenerative medicine to stimulate stem cell proliferation, to activate immune cells, and to recover wounds.
AuthorsJeongyeon Park, Donghwa Suh, Tianyu Tang, Hae June Lee, Jae-Seok Roe, Gyoo Cheon Kim, Sungbum Han, Kiwon Song
JournalFree radical biology & medicine (Free Radic Biol Med) Vol. 148 Pg. 108-122 (02 20 2020) ISSN: 1873-4596 [Electronic] United States
PMID31883975 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Chemical References
  • Cytokines
  • Intercellular Signaling Peptides and Proteins
  • Plasma Gases
Topics
  • Adipose Tissue
  • Adult
  • Cell Proliferation
  • Cytokines (genetics)
  • Epigenesis, Genetic
  • Humans
  • Intercellular Signaling Peptides and Proteins (genetics)
  • Mesoderm
  • Plasma Gases
  • Stem Cells

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