We measured the specific
8-oxo-dGTPase activity profile of the NCI-60 panel of malignant cell lines, and MTH1
protein levels in a subset of 16 lines. Their
8-oxo-dGTPase activity was compared to twelve publicly accessible MTH1
mRNA expression data bases and their cross-consistency was analyzed.
8-oxo-dGTPase and MTH1
protein levels in these cell lines are generally, but not always, mainly determined by MTH1
mRNA expression levels. The
aneuploidy number of MTH1 gene copies only slightly affects its
mRNA expression levels. By using the data mining platforms Compare and CellMiner, our
8-oxo-dGTPase profile was compared to five global gene expression datasets to identify genes whose expression levels are directly or inversely associated with
8-oxo-dGTPase. We analyzed effects of SNP within MTH1 on MTH1
mRNA level and
enzyme activity. Similar association analysis was performed for five
microRNA expression datasets. We identified several
proteins and
microRNA which might be involved in the regulation of MTH1 expression and we discuss potential mechanisms. Comparison of chemical and natural products sensitivities of the NCI-60 panel suggests seven compounds which are directly or inversely associated with
8-oxo-dGTPase. We provide an integrated picture of MTH1 expression combined from eleven consistent MTH1
mRNA and our
8-oxo-dGTPase activity NCI-60 profiles.