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Elucidating the active δ-opioid receptor crystal structure with peptide and small-molecule agonists.

Abstract
Selective activation of the δ-opioid receptor (DOP) has great potential for the treatment of chronic pain, benefitting from ancillary anxiolytic and antidepressant-like effects. Moreover, DOP agonists show reduced adverse effects as compared to μ-opioid receptor (MOP) agonists that are in the spotlight of the current "opioid crisis." Here, we report the first crystal structures of the DOP in an activated state, in complex with two relevant and structurally diverse agonists: the potent opioid agonist peptide KGCHM07 and the small-molecule agonist DPI-287 at 2.8 and 3.3 Å resolution, respectively. Our study identifies key determinants for agonist recognition, receptor activation, and DOP selectivity, revealing crucial differences between both agonist scaffolds. Our findings provide the first investigation into atomic-scale agonist binding at the DOP, supported by site-directed mutagenesis and pharmacological characterization. These structures will underpin the future structure-based development of DOP agonists for an improved pain treatment with fewer adverse effects.
AuthorsTobias Claff, Jing Yu, Véronique Blais, Nilkanth Patel, Charlotte Martin, Lijie Wu, Gye Won Han, Brian J Holleran, Olivier Van der Poorten, Kate L White, Michael A Hanson, Philippe Sarret, Louis Gendron, Vadim Cherezov, Vsevolod Katritch, Steven Ballet, Zhi-Jie Liu, Christa E Müller, Raymond C Stevens
JournalScience advances (Sci Adv) Vol. 5 Issue 11 Pg. eaax9115 (11 2019) ISSN: 2375-2548 [Electronic] United States
PMID31807708 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
Chemical References
  • OPRM1 protein, human
  • Peptides
  • Receptors, Opioid, delta
  • Receptors, Opioid, mu
Topics
  • Animals
  • Crystallography, X-Ray
  • Humans
  • Molecular Docking Simulation
  • Peptides (chemistry)
  • Protein Domains
  • Receptors, Opioid, delta (agonists, chemistry)
  • Receptors, Opioid, mu (agonists, chemistry)
  • Sf9 Cells
  • Spodoptera

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