Adipokinetic hormone promotes infection with entomopathogenic fungus Isaria fumosorosea in the cockroach Periplaneta americana.

Abstract

The adipokinetic hormones (AKHs) are known to be involved in insect immunity, thus their role in the cockroach Periplaneta americana infected with the entomopathogenic fungus Isaria fumosorosea was examined in this study. The application of I. fumosorosea resulted in a significant increase in both Akh gene expression and AKH peptide levels. Further, co-application of I. fumosorosea with Peram-CAH-II significantly enhanced cockroach mortality compared with the application of I. fumosorosea alone. The mechanism of AKH action could involve metabolic stimulation, which was indicated by a significant increase in carbon dioxide production; this effect can increase the turnover and thus efficacy of toxins produced by I. fumosorosea in the cockroach's body. I. fumosorosea treatment resulted in a significant decrease in haemolymph nutrients (carbohydrates and lipids), but co-application with Peram-CAH-II restored control level of lipids or even further increased the level of carbohydrates. Such nutritional abundance could enhance the growth and development of I. fumosorosea. Further, both I. fumosorosea and Peram-CAH-II probably affected oxidative stress: I. fumosorosea alone curbed the activity of catalase in the cockroach's gut, but co-application with Peram-CAH-II stimulated it. Interestingly, the hormone alone had no effect on catalase activity. Taken together, the results of the present study demonstrate the interactions between the fungus and AKH activity; understanding this relationship could provide insight into AKH action and may have practical implications for insect pest control in the future.

• Authors: Umesh Kumar Gautam, Andrea Bohatá, Haq Abdul Shaik, Rostislav Zemek, Dalibor Kodrík
• Journal: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP (Comp Biochem Physiol C Toxicol Pharmacol) Vol. 229 Pg. 108677 (Mar 2020) ISSN: 1532-0456 [Print] United States
• PMID: 31783176 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Inc. All rights reserved.

Chemical References

• Insect Hormones
• Oligopeptides
• adipokinetic hormone
• Carbon Dioxide
• Catalase
• Pyrrolidonecarboxylic Acid

Topics

• Animals
• Carbon Dioxide (metabolism)
• Catalase (metabolism)
• Insect Control (methods)
• Insect Hormones (pharmacology)
• Oligopeptides (pharmacology)
• Oxidative Stress
• Periplaneta (drug effects)
• Pyrrolidonecarboxylic Acid (analogs & derivatives, pharmacology)