Poly (hexamethylene biguanide), adsorbed onto Ti-Al-V alloys, kills slime-producing Staphylococci and Pseudomonas aeruginosa without inhibiting SaOs-2 cell differentiation.

Abstract

Antimicrobial coating of implant material with poly(hexamethylene biguanide) hydrochloride (PHMB) may be an eligible method for preventing implant-associated infections. In the present study, an antibacterial effective amount of PHMB is adsorbed on the surface of titanium alloy after simple chemical pretreatment. Either oxidation with 5% H2 O2 for 24 hr or processing for 2 hr in 5 M NaOH provides the base for the subsequent formation of a relatively stable self-assembled PHMB layer. Compared with an untreated control group, adsorbed PHMB produces no adverse effects on SaOs-2 cells within 48 hr cell culture, but promotes the initial attachment and spreading of the osteoblasts within 15 min. Specimens were inoculated with slime-producing bacteria to simulate a perioperative infection. Adsorbed PHMB reacts bactericidally against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa after surface contact. Adhered SaOs-2 cells differentiate and produce alkaline phosphatase and deposit calcium within 4 days in a mineralization medium on PHMB-coated Ti6Al4V surfaces, which have been precontaminated with S. epidermidis. The presented procedures provide a simple method for generating biocompatibly and antimicrobially effective implant surfaces that may be clinically important.

• Authors: Melanie Hornschuh, Paula Zwicker, Thomas Schmidt, Birgit Finke, Axel Kramer, Gerald Müller
• Journal: Journal of biomedical materials research. Part B, Applied biomaterials (J Biomed Mater Res B Appl Biomater) Vol. 108 Issue 5 Pg. 1801-1813 (07 2020) ISSN: 1552-4981 [Electronic] United States
• PMID: 31774237 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc.

Chemical References

• Alloys
• Anti-Bacterial Agents
• Biguanides
• Coated Materials, Biocompatible
• titanium alloy (TiAl6V4)
• polihexanide
• Titanium
• Alkaline Phosphatase

Topics

• Alkaline Phosphatase (metabolism)
• Alloys (chemistry)
• Anti-Bacterial Agents (chemistry, pharmacology)
• Biguanides (chemistry, pharmacology)
• Cell Adhesion
• Cell Differentiation
• Cell Line
• Cell Proliferation
• Coated Materials, Biocompatible (chemistry, metabolism)
• Humans
• Osteoblasts (cytology)
• Prostheses and Implants
• Pseudomonas aeruginosa (drug effects)
• Staphylococcus (drug effects)
• Surface Properties
• Titanium (chemistry)