Downregulation of lncRNA SNHG12 reversed IGF1R-induced osteosarcoma metastasis and proliferation by targeting miR-195-5p.

Abstract

Long non-coding RNA SNHG12 (lncSNHG12) plays important roles in the onset and progression of various cancers. However, the role of lncSNHG12 in osteosarcoma (OS) remains unclear. Therefore, the aim of the present study was to determine the function of lncSNHG12 in OS. A bioinformatics website was used to predict the downstream targets of lncSNHG12. In addition, qRT-PCR was employed to assess lncSNHG12 expression in OS cells. Cell migration and proliferation in vitro were verified using the transwell migration, clone formation, and CCK8 assays. Tumor metastasis and xenograft formation were monitored in nude mice with or without downregulation of lncSNHG12. The results show that lncSNHG12 was upregulated in OS cell lines. Downregulation lncSNHG12 suppressed the metastasis and proliferation both in vitro and in vivo. Also, lncSNHG12 downregulation suppressed the expression of insulin growth factor 1 receptor (IGF1R) expression through sponging miR-195-5p, which was verified with the luciferase reporter assay and rescue experiments. These findings suggest that downregulation of lncSNHG12 may suppress aggressive OS phenotypes. Moreover, lncSNHG12 silencing inhibited OS metastasis and growth by targeting the miR-195-5p/IGF1R axis, which represents a candidate marker and target for OS treatment and management.

• Authors: Ning Xu, Jiayuan Xu, Zhuan Zuo, Yang Liu, Feng Yan, Chenglong Han
• Journal: Gene (Gene) Vol. 726 Pg. 144145 (Feb 05 2020) ISSN: 1879-0038 [Electronic] Netherlands
• PMID: 31743769 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• IGF1R protein, human
• MIRN195 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• SNHG12 long non-coding RNA, human
• Receptor, IGF Type 1

Topics

• Animals
• Bone Neoplasms (genetics, pathology)
• Cell Line
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Disease Progression
• Down-Regulation (genetics)
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• MicroRNAs (genetics)
• Neoplasm Metastasis (genetics)
• Osteoblasts (pathology)
• Osteosarcoma (genetics, pathology)
• Prognosis
• RNA, Long Noncoding (genetics)
• Receptor, IGF Type 1 (genetics)
• Up-Regulation (genetics)