Aptamer recognition and proximity-induced entropy-driven circuit for enzyme-free and rapid amplified detection of platelet-derived growth factor-BB.

Abstract

Platelet-derived growth factor-BB (PDGF-BB) is currently used as a biomarker protein for cancer early diagnosis and clinical treatment. Herein, we reported a robust and enzyme-free strategy based on aptamer recognition and proximity-induced entropy-driven circuits (AR-PEDC) for homogeneous and rapid detection of platelet-derived growth factor BB (PDGF-BB) without any washing steps or thermocycling. The proximity probes specifically recognize target protein to form the completed trigger (CT). Then, the CT reacts with three-strand complex to form intermediate, which subsequently binds to fuel strand to release reporter strand, assistant strand and the CT. The revised proximity probes exhibit significantly improved signal-to-background ratio and faster association rate. Moreover, target protein/proximity probes interaction can specifically initiate entropy-driven circuits, thus providing immense signal amplification for ultrasensitive detection of PDGF-BB with low detection limit of 9.6 pM. The practical ability of the developed strategy is demonstrated by detection of PDGF-BB in human serum with satisfactory results. In addition, this method is flexible and can be conveniently extended to a variety of targets by simply substituting the target specific sequence. Thus, this strategy presents a rapid, low background and versatile amplification mechanism for the detection of protein biomarkers and offers a promising alternative platform for clinical diagnosis.

• Authors: Dandan Li, Xinmin Li, Bo Shen, Pu Li, Yuanjiao Chen, Shijia Ding, Weixian Chen
• Journal: Analytica chimica acta (Anal Chim Acta) Vol. 1092 Pg. 102-107 (Dec 27 2019) ISSN: 1873-4324 [Electronic] Netherlands
• PMID: 31708022 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Alkanesulfonates
• Aptamers, Nucleotide
• Azo Compounds
• BHQ-1 compound
• Biomarkers, Tumor
• Fluoresceins
• Fluorescent Dyes
• Becaplermin
• DNA

Topics

• Alkanesulfonates (chemistry)
• Aptamers, Nucleotide (chemistry, genetics)
• Azo Compounds (chemistry)
• Becaplermin (blood)
• Biomarkers, Tumor (blood)
• Biosensing Techniques (methods)
• DNA (chemistry, genetics)
• Fluoresceins (chemistry)
• Fluorescent Dyes (chemistry)
• Humans
• Limit of Detection
• Nucleic Acid Hybridization
• Reproducibility of Results
• Spectrometry, Fluorescence (methods)