Objective: To investigate the change in expression of anti-senescence marker
protein calmodulin (RGN) in liver tissues of rats with immune hepatic
fibrosis, and to observe the effect of compound
glutathione inosine injection (CGII) on it. Methods: Rat
liver fibrosis model was induced by
intraperitoneal injection of porcine serum, and CGII intervention was administered at the appropriate time. Rat liver tissues were stained with HE and Masson. RGN and
protein expression at
mRNA in liver tissues was detected by fluorescence quantitative PCR and immunohistochemistry. One-way Anova was used for measurement data. LDS test was used for two-way comparison, and pathological semi-quantitative results were analyzed by rank-sum test. Results: The relative expression of RGN
mRNA and
protein in liver tissue of fibrotic rats was 82.23 ± 15.21 and 12.52 ± 3.23, respectively, which were significantly lower than that of normal rats 176.39 ± 11.35 and 59.23 ± 9.13 (P < 0.01). The degree of
liver fibrosis in fibrotic rats after CGII intervention was significantly lower than fibrotic rats. The relative expression of RGN
mRNA and
protein in the intervention group was 168.78 ± 21.31 and 46.42 ± 4.71, respectively, which were significantly higher than
fibrosis and spontaneous recovery group. The difference was statistically significant (P < 0.01). The relative expression of RGN
mRNA and
protein in the spontaneous recovery group was 86.23 ± 17.16 and 14.34 ± 5.16, which was higher than model group. The difference was not statistically significant (P > 0.05). Conclusion: The expression of RGN in liver tissue of rats with hepatic
fibrosis induced by porcine serum is decreased, while the expression of RGN increases with the decrease of
fibrosis after CGII intervention, suggesting that the
protein may play an important role in the development of
liver fibrosis.