Abstract |
Since the prognosis of hypopharyngeal squamous cell carcinoma (HSCC) remains poor, identification of miRNA as a potential prognostic biomarker for HSCC may help improve personalized therapy. In the 2 cohorts with a total of 511 patients with HSCC (discovery: N = 372 and validation: N = 139) after post-operative radiotherapy, we used miRNA microarray and qRT-PCR to screen out the significant miRNAs which might predict survival. Associations of miRNAs and the signature score of these miRNAs with survival were performed by Kaplan-Meier survival analysis and multivariate Cox hazard model. Among 9 candidate, miRNAs, miR-200a-3p, miR-30b-5p, miR-3161, miR-3605-5p, miR-378b and miR-4451 were up-regulated, while miR-200c-3p, miR-429 and miR-4701 were down-regulated after validation. Moreover, the patients with high expression of miR-200a-3p, miR-30b-5p and miR-4451 had significantly worse overall survival (OS) and disease-specific survival (DSS) than did those with low expression (log-rank P < .05). Patients with a high-risk score had significant worse OS and DSS than those with low-risk score. Finally, after adjusting for other important prognostic confounders, patients with high expression of miR-200a-3p, miR-30b-5p and miR-4451 had significantly high risk of overall death and death owing to HSCC and patients with a high-risk score has approximately 2-fold increased risk in overall death and death owing to HSCC compared with those with a low-risk score. These findings indicated that the 3-miRNA-based signature may be a novel independent prognostic biomarker for patients given surgery and post-operative radiotherapy, supporting that these miRNAs may jointly predict survival of HSCC.
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Authors | Xinbo Xu, Zhongming Lu, Neil Gross, Guojun Li, Fenghua Zhang, Dapeng Lei, Xinliang Pan |
Journal | Journal of cellular and molecular medicine
(J Cell Mol Med)
Vol. 23
Issue 12
Pg. 8280-8291
(12 2019)
ISSN: 1582-4934 [Electronic] England |
PMID | 31578816
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2019 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. |
Chemical References |
- Biomarkers, Tumor
- MIRN200 microRNA, human
- MIRN30b microRNA, human
- MicroRNAs
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Topics |
- Biomarkers, Tumor
(genetics)
- Carcinoma, Squamous Cell
(radiotherapy, surgery)
- Combined Modality Therapy
- Female
- Gene Expression Profiling
(methods)
- Gene Expression Regulation, Neoplastic
- Humans
- Hypopharyngeal Neoplasms
(radiotherapy, surgery)
- Kaplan-Meier Estimate
- Male
- MicroRNAs
(genetics)
- Middle Aged
- Prognosis
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