Sulfonated chitosan oligosaccharide alleviates the inhibitory effect of basic fibroblast growth factor on osteogenic differentiation of human periodontal ligament stem cells.

Abstract

BACKGROUND:

Periodontal ligament stem cells (PDLSCs) play an essential role in periodontal tissue repair. Basic fibroblast growth factor (bFGF) has been used in the clinical treatment of periodontal disease. However, studies have shown that bFGF inhibits the osteogenic differentiation of PDLSCs, which is not conducive to alveolar bone repair. Sulfonated chitosan oligosaccharide (SCOS), a heparan-like compound, can maintain the conformation of bFGF and promote its proliferation activity. This study investigated the effects of bFGF in combination with SCOS on the osteogenic differentiation of hPDLSCs.

METHODS:

hPDLSCs were isolated from healthy human periodontal ligament and identified by flow cytometry and immunofluorescence. The affinity between SCOS and bFGF was analyzed by surface plasmon resonance. Changes in osteogenic differentiation by combination of bFGF with SCOS were analyzed by alkaline phosphatase activity assay, Sirius Red staining, and Alizarin Red staining. Expression of genes and proteins was investigated by western blotting and reverse transcription-quantitative PCR.

RESULTS:

Extracted hPDLSCs were mesenchymal stem cells with pluripotent differentiation potential. SCOS exhibited an affinity for bFGF. bFGF (20 ng/mL) promoted the proliferation of hPDLSCs, but inhibited their osteogenic differentiation. SCOS alleviated the inhibitory effect of bFGF on the osteogenic differentiation of hPDLSCs.

CONCLUSIONS:

SCOS can reduce the inhibitory effect of bFGF on the osteogenic differentiation of hPDLSCs. This study provides evidence for the clinical use of bFGF to repair periodontal tissue.

• Authors: Yangfan Li, Fenglin Yu, Yang Liu, Qian Liang, Yadong Huang, Qi Xiang, Qihao Zhang, Zhijian Su, Yan Yang, Yueping Zhao
• Journal: Journal of periodontology (J Periodontol) Vol. 91 Issue 7 Pg. 975-985 (07 2020) ISSN: 1943-3670 [Electronic] United States
• PMID: 31573683 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 American Academy of Periodontology.

Chemical References

• Oligosaccharides
• Fibroblast Growth Factor 2
• Chitosan

Topics

• Cell Differentiation
• Cell Proliferation
• Cells, Cultured
• Chitosan (pharmacology)
• Fibroblast Growth Factor 2 (pharmacology)
• Humans
• Oligosaccharides
• Osteogenesis
• Periodontal Ligament
• Stem Cells