Abstract |
In this work, the authors investigated the apoptotic activities of Fe3O4/Ag nanocomposite biosynthesised by Spirulina platensis extract against MCF-7 (human breast cancer cells). The physico-chemical properties of prepared Fe3O4/Ag nanocomposite were studied by different spectroscopic methods. To evaluate the in vitro cytotoxic effect, MCF-7 cells were treated with different concentrations of Fe3O4/Ag nanocomposite and examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Moreover, apoptotic effects were also studied by Hoechst 33258 staining, caspase 3 activation assays, and annexin V- fluorescein isothiocyanate ( FITC) and propidium iodide staining. Microscopic observations of Fe3O4/Ag nanocomposites indicated approximately spherical shape and small particles in the size range of about 30-50 nm. The MTT assay result revealed that the Fe3O4/Ag nanocomposite causes a dose-dependent cell proliferation reduction in MCF-7 cells (IC50 = 135 μg/ml). Regarding to the Annexin V/PI staining result, the increase percentage of apoptotic cells (28.09%) was detected as compared to untreated cells. According to the caspase assay, Fe3O4/Ag nanocomposite enhances caspase 3 level. Furthermore, in vitro anti- cancer activity of the nanocomposite was performed by Hoechst 33258 staining method. The proposed data suggest that Fe3O4/Ag nanocomposite may be an effective agent for the inhibition of breast cancer cells at in vitro level.
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Authors | Ali Salehzadeh, Akram Sadat Naeemi, Ladan Khaknezhad, Zeinab Moradi-Shoeili, Seyed Ataollah Sadat Shandiz |
Journal | IET nanobiotechnology
(IET Nanobiotechnol)
Vol. 13
Issue 7
Pg. 766-770
(Sep 2019)
ISSN: 1751-875X [Electronic] United States |
PMID | 31573547
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Plant Extracts
- Silver
- Ferrosoferric Oxide
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Topics |
- Antineoplastic Agents
(chemical synthesis, chemistry, pharmacology)
- Apoptosis
(drug effects)
- Cell Proliferation
(drug effects)
- Drug Screening Assays, Antitumor
- Ferrosoferric Oxide
(chemistry)
- Humans
- MCF-7 Cells
- Materials Testing
- Metal Nanoparticles
(chemistry, therapeutic use)
- Nanocomposites
(chemistry, therapeutic use)
- Plant Extracts
(chemistry, metabolism, pharmacology)
- Silver
(chemistry)
- Spirulina
(chemistry, metabolism)
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