Curcumin protects the pancreas from acute pancreatitis via the mitogen‑activated protein kinase signaling pathway.

Abstract

Curcumin has been demonstrated to reduce markers of inflammation during acute pancreatitis (AP). However, the underlying mechanisms of the protective effects of curcumin are unknown. In the present study the effects of curcumin in an AP animal model and cell models was examined and the underlying mechanisms were investigated. An AP animal model was established by injection of 5% sodium taurocholate into the biliopancreatic duct of rats, and the cell model was established by treatment with 0.5 nM cerulein with an optimal concentration of lipopolysaccharide in AR42J rat pancreatic cancer cells. Amylase activity and arterial blood gas composition were assessed by automatic biochemical and blood gas analyzers. Pathological alteration of the pancreas was determined by hematoxylin and eosin staining. Interleukin (IL‑6), tumor necrosis factor (TNF)‑α and C‑reactive protein (CRP) levels were measured by ELISA. Cell viability was determined by Cell Counting Kit‑8 and protein expression levels were assessed by western blotting. Curcumin reduced the ascites volume after 12 and 24 h, the weight of pancreas after 12, 24 and 36 h of surgery, but also attenuated injury to the pancreas. Serum expression levels of TNF‑α and CRP were reduced by curcumin. In addition, curcumin decreased the cell viability, amylase activity and the phosphorylation of p38 in AR42J cells, but did not affect the intracellular levels of IL‑6 and TNF‑α. Curcumin may lower the severity and inflammatory response via the mitogen‑activated protein kinase‑signaling pathway, to some extent. However, future studies are required to fully understand the protective effects of curcumin on AP.

• Authors: Yingjie Wang, Chanyuan Bu, Kangkang Wu, Rui Wang, Jiayong Wang
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 20 Issue 4 Pg. 3027-3034 (Oct 2019) ISSN: 1791-3004 [Electronic] Greece
• PMID: 31432122 (Publication Type: Journal Article)

Chemical References

• Il6 protein, rat
• Interleukin-6
• Tumor Necrosis Factor-alpha
• C-Reactive Protein
• Curcumin

Topics

• Animals
• C-Reactive Protein (metabolism)
• Cell Line, Tumor
• Curcumin (pharmacology)
• Disease Models, Animal
• Female
• Interleukin-6 (metabolism)
• MAP Kinase Signaling System (drug effects)
• Pancreas (metabolism, pathology)
• Pancreatitis (metabolism, pathology, prevention & control)
• Rats
• Rats, Sprague-Dawley
• Tumor Necrosis Factor-alpha (metabolism)