The aim of this paper was to investigate the mechanism and effect of
psoralen and
isopsoralen in the treatment of
lipid accumulation in LO2 cells. Human LO2 cells
nonalcoholic fatty liver models were established by using
palmitic acid( PA). Then
psoralen and
isopsoralen were administered for intervention. Intracellular
triglyceride( TG) and total
cholesterol( TC) content,the cell supernatant
alanine aminotransferase( ALT) and
aspartate aminotransferase( AST) levels were determined by
enzyme method. Cell supernatant proinflammatory cytokines( IL-6,TNF-α) and chemokines( IL-8,MCP-1) were determined by ELISA method. Western blot method was conducted to detect the
protein expression of intracellular nuclear factor( NF-κB) p65 phosphorylation( p-p65),nonphosphorylated
protein( p65),and transforming factor TGF-β1. Result showed that as compared with the model group,intracellular TG and TC levels,the cell supernatant ALT and AST levels,proinflammatory
cytokines and
chemokines were decreased( P < 0. 01,P <0. 05); the p-p65/p65 ratio and TGF-β1
protein expression were also significantly decreased( P< 0. 01,P< 0. 05) in
psoralen intervention group. As compared with the model cells,intracellular TG content had no significant changes,but all the other indexes were reduced( P<0. 01,P<0. 05) in the cells of
isopsoralen intervention group.
Psoralen exhibited better effect than
isopsoralen( P< 0. 01,P<0. 05). It is concluded that
psoralen could improve the adipogenesis of LO2 cells induced by PA; both
psoralen and
isopsoralen are effective in ameliorating LO2 cells injury induced by PA,reducing
inflammation via inhibiting the activation of NF-κB and down-regulating the expression of TGF-β1.