Abstract | OBJECTIVE: METHODS: Highly purified p40 protein was used to quantitate IgG autoantibodies in serum from 172 SLE patients and from disease controls and age-matched healthy subjects by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Preparations of p40 that also contained associated proteins were analyzed by immunoblotting with patient sera. RESULTS:
Antibodies reactive with p40 were detected in the majority of patients and many healthy controls. Their levels were higher in patients with SLE, but not those with systemic sclerosis, compared to healthy subjects (P = 0.01). Anti-p40 reactivity was higher in patients during a flare than in patients with disease in remission (P = 0.03); correlated with the SLE Disease Activity Index score (P = 0.0002), type I interferon score (P = 0.006), decrease in complement C3 level (P = 0.0001), the presence of anti-DNA antibodies (P < 0.0001) and anti-C1q antibodies (P = 0.004), and current or past history of nephritis (P = 0.02 and P = 0.003, respectively); and correlated inversely with age (r = -0.49, P < 0.0001). SLE patient sera also reacted with p40-associated proteins. CONCLUSION:
Autoantibodies reacting with LINE-1 p40 characterize a population of SLE patients with severe and active disease. These autoantibodies may represent an early immune response against LINE-1 p40 that does not yet by itself imply clinically significant autoimmunity, but may represent an early, and still reversible, step toward SLE pathogenesis.
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Authors | Victoria Carter, John LaCava, Martin S Taylor, Shu Ying Liang, Cecilia Mustelin, Kennedy C Ukadike, Anders Bengtsson, Christian Lood, Tomas Mustelin |
Journal | Arthritis & rheumatology (Hoboken, N.J.)
(Arthritis Rheumatol)
Vol. 72
Issue 1
Pg. 89-99
(01 2020)
ISSN: 2326-5205 [Electronic] United States |
PMID | 31342656
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2019, American College of Rheumatology. |
Chemical References |
- Antibodies, Antinuclear
- Autoantibodies
- Complement C3
- DNA-Binding Proteins
- L1Hs-encoded protein p40, human
- Complement C1q
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Topics |
- Age Factors
- Antibodies, Antinuclear
(immunology)
- Autoantibodies
(immunology)
- Case-Control Studies
- Complement C1q
(immunology)
- Complement C3
(immunology)
- DNA-Binding Proteins
(immunology)
- Humans
- Long Interspersed Nucleotide Elements
(immunology)
- Lupus Erythematosus, Systemic
(immunology)
- Lupus Nephritis
(immunology)
- Scleroderma, Systemic
(immunology)
- Severity of Illness Index
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