Foodborne Botulism Outbreak Associated With Commercial Nacho Cheese Sauce From a Gas Station Market.

Abstract

BACKGROUND:

Botulism is a rare and potentially fatal paralytic disease caused by botulinum neurotoxin (BoNT). In April 2017, 4 California residents from 2 adjacent counties were hospitalized with suspected foodborne botulism, precipitating an investigation by state and local public health departments in California.

METHODS:

We interviewed suspected botulism patients and their families, inspected the suspect establishment, and collected suspect food. We tested patient sera, stool, and gastric aspirates using mouse bioassay for BoNT and/or culture for Clostridium botulinum. We tested suspect food and environmental samples for BoNT and confirmed presumptive positives using direct mouse bioassay and culture. We performed whole-genome sequencing on food and clinical isolates.

RESULTS:

From April 2017 through May 2017, 10 patients in the Sacramento area were hospitalized with laboratory-confirmed botulism; 7 required mechanical ventilation, and 1 died. Of 9 patients with information, all had visited Gas Station X before illness onset, where 8 reported consuming a commercial cheese sauce. BoNT/A and/or BoNT/A-producing C. botulinum were detected from each patient and from leftover cheese sauce. Clostridium botulinum isolates from 4 patients were closely related to cheese sauce isolates by whole-genome high-quality single-nucleotide polymorphism analysis. No other botulism cases associated with this cheese sauce were reported elsewhere in the United States.

CONCLUSIONS:

This large foodborne botulism outbreak in California was caused by consumption of commercial cheese sauce dispensed at a gas station market. The epidemiologic and laboratory evidence confirmed the cheese sauce as the outbreak source. The cheese sauce was likely locally contaminated, although the mechanism is unclear.

• Authors: Hilary E Rosen, Akiko C Kimura, John Crandall, Alyssa Poe, June Nash, Jason Boetzer, Selam Tecle, Rituparna Mukhopadhyay, Kate Mcauley, Olivia Kasirye, Alvaro Garza, Mahtab Shahkarami, Vishnu Chaturvedi, David Kiang, Jeff Vidanes, Kelly Mccoy, Mark Barcellos, Tammy Derby, Seema Jain, Duc J Vugia
• Journal: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America (Clin Infect Dis) Vol. 70 Issue 8 Pg. 1695-1700 (04 10 2020) ISSN: 1537-6591 [Electronic] United States
• PMID: 31247064 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].

Topics

• Animals
• Botulism (epidemiology)
• Cheese
• Clostridium botulinum (genetics)
• Disease Outbreaks
• Humans
• Mice
• Public Health