Skullcapflavone I protects cardiomyocytes from hypoxia-caused injury through up-regulation of lincRNA-ROR.

Abstract

Myocardial infarction (MI) is a serious heart disease in which cardiomyocytes are damaged, caused by hypoxia. This study explored the possible protective activity of Skullcapflavone I (SF I), a flavonoid isolated from the root of Scutellaria baicalensis Georgi, on hypoxia-stimulated cardiomyocytes cell injury in vitro. Viability and apoptosis of H9c2 cells and primary cardiomyocytes were tested using cell counting kit-8 (CCK-8) assay and Guava Nexin Reagent, respectively. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to measure the long non-coding RNA regulator of reprogramming (lincRNA-ROR) expression. si-ROR was transfected to knockdown lincRNA-ROR. Western blotting was conducted to assess the protein levels of key molecules related to cell proliferation, apoptosis, and mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK/ERK) pathway. We discovered that hypoxia stimulation obviously reduced H9c2 cell and primary cardiomyocytes' viability and proliferation, but promoted cell apoptosis. SF I treatment mitigated the cell viability and proliferation inhibition, as well as cell apoptosis caused by hypoxia. Moreover, SF I promoted the hypoxia-caused up-regulation of lincRNA-ROR in H9c2 cells and primary cardiomyocytes. Knockdown of lincRNA-ROR reversed the influence of SF I on hypoxia-stimulated H9c2 cells and primary cardiomyocytes. Besides, SF I activated MEK/ERK pathway in H9c2 cells and primary cardiomyocytes via up-regulating lincRNA-ROR. To sum up, our research verified the beneficial activity of SF I on hypoxia-caused cardiomyocytes injury. SF I protected cardiomyocytes from hypoxia-caused injury through up-regulation of lincRNA-ROR and activation of MEK/ERK pathway.

• Authors: Zhenxiao Zhang, Hui Li, Mingyang Liu, Jianshuai He, Xiaotian Zhang, Yuehua Chen
• Journal: International journal of immunopathology and pharmacology (Int J Immunopathol Pharmacol) 2019 Jan-Dec Vol. 33 Pg. 2058738419857537 ISSN: 2058-7384 [Electronic] England
• PMID: 31220954 (Publication Type: Journal Article, Retracted Publication)

Chemical References

• Cardiotonic Agents
• Flavones
• RNA, Long Noncoding
• skullcapflavone I
• Mitogen-Activated Protein Kinases

Topics

• Animals
• Apoptosis (drug effects)
• Cardiotonic Agents (pharmacology)
• Cell Hypoxia (genetics)
• Cell Line
• Flavones (pharmacology)
• Mitogen-Activated Protein Kinases (metabolism)
• Myocytes, Cardiac (drug effects, metabolism)
• RNA, Long Noncoding
• Rats, Wistar
• Up-Regulation (drug effects)